Genotyping and primer details


Genotyping and primer details for SALK_129238c - At4g03153
Line ID SALK_129238c
Locus At4g03153 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1395095 of line SALK_129238c Chr4:1395095
   
Insertion allele amplicon LBb3 - 31fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31fx: 5'-GTGCAAAATTACAAGTGAAGACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1395611
   
wt amplicon (over InsPos) 02hb - 31fx
wt amplicon start Chr4:1394605
wt amplicon end Chr4:1395611
wt amplicon size 1007 bp
Locus specific primer 31fx: 5'-GTGCAAAATTACAAGTGAAGACGA-3'
2nd locus specific primer 02hb: 5'-ATTTGAGCGAGGAAGATAAGATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11