Genotyping and primer details


Genotyping and primer details for SALK_129340c - At3g02070
Line ID SALK_129340c
Locus At3g02070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:362543 of line SALK_129340c Chr3:362543
   
Insertion allele amplicon LBb3 - 52iv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52iv: 5'-ACATTTCCAAAGGACAAAACAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:363026
   
wt amplicon (over InsPos) 02ph - 52iv
wt amplicon start Chr3:362100
wt amplicon end Chr3:363026
wt amplicon size 927 bp
Locus specific primer 52iv: 5'-ACATTTCCAAAGGACAAAACAAAC-3'
2nd locus specific primer 02ph: 5'-CTTATGAGTTCTGACCGATGAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11