Genotyping and primer details


Genotyping and primer details for SALK_129379 - At4g02570
Line ID SALK_129379
Locus At4g02570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1132951 of line SALK_129379 Chr4:1132951
   
Insertion allele amplicon LBb3 - 98by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 98by: 5'-ACAGTTTCGAGGATTATCTAGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1132320
   
wt amplicon (over InsPos) 19da - 98by
wt amplicon start Chr4:1132320
wt amplicon end Chr4:1133112
wt amplicon size 793 bp
Locus specific primer 98by: 5'-ACAGTTTCGAGGATTATCTAGGCA-3'
2nd locus specific primer 19da: 5'-GACTTTCTTCCTTTCATCAACTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11