Genotyping and primer details


Genotyping and primer details for SALK_131274c - At3g05240
Line ID SALK_131274c
Locus At3g05240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1493787 of line SALK_131274c Chr3:1493787
   
Insertion allele amplicon LBb3 - 31hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31hd: 5'-TTTTGTTTTCGTAGCAGCAGAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1493020
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11