Genotyping and primer details


Genotyping and primer details for SALK_132358 - At3g19020
Line ID SALK_132358
Locus At3g19020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6561774 of line SALK_132358 Chr3:6561774
   
Insertion allele amplicon LBb3 - 14pt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14pt: 5'-GTTCCCAATAAACACAATCTCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6561229
   
wt amplicon (over InsPos) 02qm - 14pt
wt amplicon start Chr3:6561229
wt amplicon end Chr3:6562087
wt amplicon size 859 bp
Locus specific primer 14pt: 5'-GTTCCCAATAAACACAATCTCGTT-3'
2nd locus specific primer 02qm: 5'-TATTAAGCAAGCCATAGAGCCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11