Genotyping and primer details


Genotyping and primer details for SALK_133422c - At3g50100
Line ID SALK_133422c
Locus At3g50100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18578421 of line SALK_133422c Chr3:18578421
   
Insertion allele amplicon LBb3 - 45wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45wj: 5'-AAAACCTCTGAGTATGGCTTGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18579042
   
wt amplicon (over InsPos) 08wy - 45wj
wt amplicon start Chr3:18577717
wt amplicon end Chr3:18579042
wt amplicon size 1326 bp
Locus specific primer 45wj: 5'-AAAACCTCTGAGTATGGCTTGAAA-3'
2nd locus specific primer 08wy: 5'-ACATTGTCTTTTGGCGTCTAAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11