Genotyping and primer details


Genotyping and primer details for SALK_136932c - At1g60610
Line ID SALK_136932c
Locus At1g60610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22328203 of line SALK_136932c Chr1:22328203
   
Insertion allele amplicon LBb3 - 01bz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01bz: 5'-GAAATTGTTCTCTCTTGGAACAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22328567
   
wt amplicon (over InsPos) 32dz - 01bz
wt amplicon start Chr1:22327892
wt amplicon end Chr1:22328567
wt amplicon size 676 bp
Locus specific primer 01bz: 5'-GAAATTGTTCTCTCTTGGAACAGG-3'
2nd locus specific primer 32dz: 5'-GTTTAATGGCAAAAGCAAAGAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11