Genotyping and primer details


Genotyping and primer details for SALK_137776 - At1g76720
Line ID SALK_137776
Locus At1g76720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28798787 of line SALK_137776 Chr1:28798787
   
Insertion allele amplicon LBb3 - 63yh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63yh: 5'-TGAACTTTCTCTACTTCGGGTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28797900
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11