Genotyping and primer details


Genotyping and primer details for SALK_138998c - At3g22180
Line ID SALK_138998c
Locus At3g22180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7829970 of line SALK_138998c Chr3:7829970
   
Insertion allele amplicon LBb3 - 45vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45vi: 5'-TCAACATGCCATTTTTACACTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7829440
   
wt amplicon (over InsPos) 53vx - 45vi
wt amplicon start Chr3:7829440
wt amplicon end Chr3:7830556
wt amplicon size 1117 bp
Locus specific primer 45vi: 5'-TCAACATGCCATTTTTACACTCAT-3'
2nd locus specific primer 53vx: 5'-GACGTTTTGTAGAAGATCAGAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11