Genotyping and primer details


Genotyping and primer details for SALK_140490 - At1g03840
Line ID SALK_140490
Locus At1g03840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:968174 of line SALK_140490 Chr1:968174
   
Insertion allele amplicon LBb3 - 17kn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17kn: 5'-TCAACAGGAGAGACAGCTTCATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:968575
   
wt amplicon (over InsPos) 12mc - 17kn
wt amplicon start Chr1:967685
wt amplicon end Chr1:968575
wt amplicon size 891 bp
Locus specific primer 17kn: 5'-TCAACAGGAGAGACAGCTTCATTA-3'
2nd locus specific primer 12mc: 5'-TCCCAATATCAACTCTTCTACGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11