Genotyping and primer details


Genotyping and primer details for SALK_141443c - At1g20910
Line ID SALK_141443c
Locus At1g20910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7279872 of line SALK_141443c Chr1:7279872
   
Insertion allele amplicon LBb3 - 56dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 56dl: 5'-AATCACAGTACATGAAACCTTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7278871
   
wt amplicon (over InsPos) 06ur - 56dl
wt amplicon start Chr1:7278871
wt amplicon end Chr1:7280155
wt amplicon size 1285 bp
Locus specific primer 56dl: 5'-AATCACAGTACATGAAACCTTCCC-3'
2nd locus specific primer 06ur: 5'-GGAGGCCTTTTACAAAGAGAGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11