Genotyping and primer details


Genotyping and primer details for SALK_142629c - At4g16130
Line ID SALK_142629c
Locus At4g16130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9123880 of line SALK_142629c Chr4:9123880
   
Insertion allele amplicon LBb3 - 22gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22gg: 5'-GTCAGTGAAGCCCTATCCTACAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9123070
   
wt amplicon (over InsPos) 11ho - 22gg
wt amplicon start Chr4:9123070
wt amplicon end Chr4:9124041
wt amplicon size 972 bp
Locus specific primer 22gg: 5'-GTCAGTGAAGCCCTATCCTACAAG-3'
2nd locus specific primer 11ho: 5'-CACGTATCTGAAAGACCTTGAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11