Genotyping and primer details


Genotyping and primer details for SALK_142682c - At3g24120
Line ID SALK_142682c
Locus At3g24120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8708179 of line SALK_142682c Chr3:8708179
   
Insertion allele amplicon LBb3 - 16im
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16im: 5'-TCAAACCTGAAGATGTGATTTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8707449
   
wt amplicon (over InsPos) 01km - 16im
wt amplicon start Chr3:8707449
wt amplicon end Chr3:8708307
wt amplicon size 859 bp
Locus specific primer 16im: 5'-TCAAACCTGAAGATGTGATTTGAG-3'
2nd locus specific primer 01km: 5'-CAAACTATATCCACCCGTCTCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11