Genotyping and primer details


Genotyping and primer details for SALK_144505c - At3g15640
Line ID SALK_144505c
Locus At3g15640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5300947 of line SALK_144505c Chr3:5300947
   
Insertion allele amplicon LBb3 - 39mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39mv: 5'-ACGTCCCTATATGAAAAGACATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5301677
   
wt amplicon (over InsPos) 19oj - 39mv
wt amplicon start Chr3:5300622
wt amplicon end Chr3:5301677
wt amplicon size 1056 bp
Locus specific primer 39mv: 5'-ACGTCCCTATATGAAAAGACATCG-3'
2nd locus specific primer 19oj: 5'-GCAAGTGTCTGTAGATCATGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11