Genotyping and primer details


Genotyping and primer details for SALK_144791 - At4g17770
Line ID SALK_144791
Locus At4g17770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9877977 of line SALK_144791 Chr4:9877977
   
Insertion allele amplicon R204 - AW77
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW77: 5'-AACATATTTGTGATGCTTCTCGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9878638
   
wt amplicon (over InsPos) AW78 - AW77
wt amplicon start Chr4:9877613
wt amplicon end Chr4:9878638
wt amplicon size 1026 bp
Locus specific primer AW77: 5'-AACATATTTGTGATGCTTCTCGTG-3'
2nd locus specific primer AW78: 5'-TTTGCTGATAAAGTGATGGAAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11