Genotyping and primer details


Genotyping and primer details for SALK_147470 - At3g60880
Line ID SALK_147470
Locus At3g60880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22496156 of line SALK_147470 Chr3:22496156
   
Insertion allele amplicon LBb3 - 64gp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64gp: 5'-TGATCCATCTGTGAGTTTCTCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22496629
   
wt amplicon (over InsPos) 05il - 64gp
wt amplicon start Chr3:22495665
wt amplicon end Chr3:22496629
wt amplicon size 965 bp
Locus specific primer 64gp: 5'-TGATCCATCTGTGAGTTTCTCATC-3'
2nd locus specific primer 05il: 5'-CACCGTATTTACCTGATGGAAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11