Genotyping and primer details


Genotyping and primer details for SALK_147747c - At3g57210
Line ID SALK_147747c
Locus At3g57210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21174254 of line SALK_147747c Chr3:21174254
   
Insertion allele amplicon LBb3 - 45cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45cp: 5'-TACTCGTAGCCTTCTTTTTGGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21173288
   
wt amplicon (over InsPos) 05gi - 45cp
wt amplicon start Chr3:21173288
wt amplicon end Chr3:21174437
wt amplicon size 1150 bp
Locus specific primer 45cp: 5'-TACTCGTAGCCTTCTTTTTGGGTA-3'
2nd locus specific primer 05gi: 5'-AGCATTTAATTACAACTTGCCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11