Genotyping and primer details


Genotyping and primer details for SALK_149660c - At4g30850
Line ID SALK_149660c
Locus At4g30850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15022442 of line SALK_149660c Chr4:15022442
   
Insertion allele amplicon LBb3 - 50um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50um: 5'-TGATCAACACTCCCCATATTATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15022852
   
wt amplicon (over InsPos) 54vr - 50um
wt amplicon start Chr4:15021696
wt amplicon end Chr4:15022852
wt amplicon size 1157 bp
Locus specific primer 50um: 5'-TGATCAACACTCCCCATATTATCA-3'
2nd locus specific primer 54vr: 5'-TCTTTACGTGATGCAGAGTTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11