Sequences from confirmation amplicon


Confirmation sequences for line 395E05 and hit At1g01710 are shown.
Line ID 395E05
Locus At1g01710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:262924
Primers used for PCR 34oz - 8474, show primer details
   
Sequence Name 27-K114909-0022-395-E05C-34oz
GenBank Accession LN498515 [GenBank]
Sequencing primer gene specific primer, 34oz: 5'-ATACTGTTTCAAGAGGAATTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114909-0022-395-E05C-34oz
CAACAGAATCAAATTATAACAATGGCGAAGATAGATACATCGATATCTAGTCTCAGAAAC
AAACCTCTCCTTGCAAAATAAAATAACATCCATCCCAAGTTTGATCTTCACGAACCACAT
AATCCCCTTTCCCTTCAATAATACAATAGAGAACAACACAGCTCAATTTTTTTATATCGT
GAAATCAGAGAGAAGAATAAATCCGAAATTGAGAATGGACCGACCGTAACGTTTCGGCAC
AACAACTTGAGCAATTTTCTTCAGAGAAGAGCTCGGTAATTTCTGGAGTAAAGGGACGTT
TCCAAGGAACTCGACAACTACAAAAACAGAGCAGCAACAACATGGAATTCGCCGAAAATC
TCACTACGTCATTCCTCAATCTCACGTTATAGGCTTACCTGACTCGGTGTTCATGGCGGA
TTCGGCTAGTCGGAAGAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATT
   
Sequence Name 03-K114908-0022-395-E05C-8409
GenBank Accession LN487601 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114908-0022-395-E05C-8409
ATTTCTTCGACTGCCGAATCCGCCATGAACACCGAGTCAGGTAAGCCTATAACGTGAGAT
TGAGGAATGACGTAGTGAGATTTTCGGCGAATTCCATGTTGTTGCTGCTCTGTTTTTGTA
GTTGTCGAGTTCCTTGGAAACGTCCCTTTACTCCAGAAATTACCGAGCTCTTCTCTGAAG
AAAATTGCTCAAGTTGTTGTGCCGAAACGTTACGGTCGGTCCATTCTCAATTTCGGATTT
ATTCTTCTCTCTGATTTCACGATATAAAAAAATTGAGCTGTGTTGTTCTCTATTGTATTA
TTGAAGGGAAAGGGGATTATGTGGTTCGTGAAGATCAAACTTGGGATGGATGTTATTTTA
TTTTGCAAGGAGAGGTTTGTTTCTGAGACTAGATATCGATGTATCTATCTTCGCCATTGT
TATAATTTGATTCTTGTTTGCAGGCTCAGGTTTCCGGACCAGACGAAGAAGATAACCGTT
CCGAATTCCTCT

Last Updated on 06.12.2018 16:11