Sequences from confirmation amplicon


Confirmation sequences for line 082G07 and hit At1g02610 are shown.
Line ID 082G07
Locus At1g02610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:554110
Primers used for PCR 17nm - 8474, show primer details
   
Sequence Name 84-K117739-0022-082-G07C-17nm
GenBank Accession LN514473 [GenBank]
Sequencing primer gene specific primer, 17nm: 5'-AAAAAGAAGAGCAATTTACCGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K117739-0022-082-G07C-17nm
ACCGCATCAATGCATGCTTTATCAACAAAATAACCGAAAACTGCAAAAAGAAAAAATTAT
ATCAATTTAATTAGAGTCACGCCGTAAAAGATGTACCCATATATGGATATGTTATGATAT
GATATTTAGCTCAAATAAAATTGGTTTGGTTGAATTTGGGTATGGCTTGAATATGGGTTA
AAGCTTGGAAAAGACGTTATGGGCTATGTTACCACTTACCACTATGAACAATTTTTTAAT
TTAGTAAACAGACATTCTTTTGGTTCCAAAAGAGGAAACCTTTTACTTTCTTTAGGTTTT
CTTGGTCGACCAAACAACCTCACCGAACCACTTACCACAAATGCATGTATGTGAAAAGAA
TCCTAACAATCATTCTAATCTAATGTCATTATATTTGTCTCTATATATATTTCATAGCAC
CACCAAACTTGAACCATTTCTAACGCAAAACTATTAAAATTAATTTTTTGTTTTCGAAAC
TAACATATTAAAAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 68-K117738-0022-082-G07C-8409
GenBank Accession LN512087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K117738-0022-082-G07C-8409
AAACAAAAAATTAATTTTAATAGTTTTGCGTTAGAAATGGTTCAAGTTTGGTGGTGCTAT
GAAATATATATAGAGACAAATATAATGACATTAGATTAGAATGATTGTTAGGATTCTTTT
CACATACATGCATTTGTGGTAAGTGGTTCGGTGAGGTTGTTTGGTCGACCAAGAAAACCT
AAAGAAAGTAAAAGGTTTCCTCTTTTGGAACCAAAAGAATGTCTGTTTACTAAATTAAAA
AATTGTTCATAGTGGTAAGTGGTAACATAGCCCATAACGTCTTTTCCAAGCTTTAACCCA
TATTCAAGCCATACCCAAATTCAACCAAACCAATTTTATTTGAGCTAAATATCATATCAT
AACATATCCATATATGGGTACATCTTTTACGGCGTGACTCTAATTAAATTGATATAATTT
TTTCTTTTTGCAGTTTTCGGTTATTTTGTTGATAAAGCATGCATTTGATGCGGTTTATGG
AACTGAAGAGTATCCCTATACAATATTCACGGTAAATTGCTCTTCTTTTT

Last Updated on 06.12.2018 16:11