Sequences from confirmation amplicon


Confirmation sequences for line 677H05 and hit At1g02830 are shown.
Line ID 677H05
Locus At1g02830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:625647
Primers used for PCR CQ73 - 8474, show primer details
   
Sequence Name 26-K107131-0022-677-H05C-CQ73
GenBank Accession LN497952 [GenBank]
Sequencing primer gene specific primer, CQ73: 5'-CCACTAAGATGAAAAACTGCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K107131-0022-677-H05C-CQ73
ACCTTGAGAGTTGTATAGATGTTTTATTCATAATTGTACAATTGTAAGAGAATAAACCAG
TGTTTGATATAGTTTCCTCCACATCTTTTAAATGCTGAGATAACTACTCTAGGAAATGAA
ATACGGTACAGGTTTTATGTGATCAAATATCTCTTGATGTTGAACAAAGATTAATTGAAC
CGAGGCCGGTTCAATATTTGATATCATTTCGGATCAAACAGAAATTAGACAAGCTCTTAG
CCCAAGTATTGGGCCTAAGAAGCCCATTGGGCTAACACTAAACAAAACCCTAGGGACTCT
TGTGGGTCATTTATATAGTCCATCCCTAACCTCGCCGCGGGAAGATTTTCATCTTTTCGA
GCCAACGTCTAATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 10-K107130-0022-677-H05C-8409
GenBank Accession LN490681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K107130-0022-677-H05C-8409
GCCATTTACATGTAGATTAGACGTTGGCTCGAAAAGATGAAAATCTTCCCGCGGCGAGGT
TAGGGATGGACTATATAAATGACCCACAAGAGTCCCTAGGGTTTTGTTTAGTGTTAGCCC
AATGGGCTTCTTAGGCCCAATACTTGGGCTAAGAGCTTGTCTAATTTCTGTTTGATCCGA
AATGATATCAAATATTGAACCGGCCTCGGTTCAATTAATCTTTGTTCAACATCAAGAGAT
ATTTGATCACATAAAACCTGTACCGTATTTCATTTCCTAGAGTAGTTATCTCAGCATTTA
AAAGATGTGGAGGAAACTATATCAAACACTGGTTTATTCTCTTACAATTGTACAATTATG
AATAAAACATCTATACAACTTCTCAAGGCTTTGCGATTTCGCATTGCGACTCGGAAGCAG
TTTTTCTNCCTTAGTGG

Last Updated on 06.12.2018 16:11