Sequences from confirmation amplicon


Confirmation sequences for line 543H08 and hit At1g02850 are shown.
Line ID 543H08
Locus At1g02850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:633111
Primers used for PCR 50zd - 8474, show primer details
   
Sequence Name 26-K124484-0022-543-H08C-50zd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 50zd: 5'-TAACTCTGTGAAGGATAAGCAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K124484-0022-543-H08C-50zd
TATGCACTTGATTCACGCATGAGTTTCTTGATTCGAGTTTATGTAAGGATTTTACATCCG
TTGGTGTTTGGTGATTATCCTGAGACGATGAAGACCAATGTTGGGTCTAGATTGCCAGCT
TTTACAGAGGAGGAGTCAGAACAAGTTAAAGGCGCATTTGATTTCGTTGGAGTGATAAAT
TACATGGCGCTTTATGTCAAAGACAACTCTTCCTCTCTGAAACCTAATCTCCAGGATTTC
AACACAGATATAGCCGTGGAAATGACCTGTAAGTTATATGATACTGTCTGAGAATTAAAA
GTTAAAAATCTGCAGGCTCACTGGTTTCTCCATTTTGCAGTGGTTGGAAACACATCAATC
GAGAATGAGGTTTGTATGTAGATATCAATTTTATATGTGATCACCTACAAGAGACAAGAC
TGATGTTTGATGATTTGCTTCTTTCAGTATGCAAATACACCATGGAGTCTGCAACAGATA
CTTTTGTATGTCAAAGAAACTTATGGAAACCCTCCTGTCTATATCCTAGAGAATGGTCTC
TCTTTATCTCTCTCTGCCTCTCTCAGACTTTAGTTTGCTTCTTCATGTGTCTTAAACTCT
CTCTCTCTGTCGGGTGATTGATTCTTCATTTACAGGACAAATGACTCCTCACAGCTCATC
ACTTGTGGACACAACAAGGGTGAAGTATCTGAGCTCGTACATTAAAGCAGTGCTTCATTC
ACTCAGGTAACAGTAAAAGTTGTTCAAGTTATCATTCAATGGATGTGTCAGTCAGATTCT
AAGCATAAAATTTGACACAGCAGGAAAGGATCGGACGTAAAAGGGTATTTTCAGTGGTCG
TTGATGGATGTGTTTGAGCTATTTGGTGGGTA
   
Sequence Name 02-K124483-0022-543-H08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K124483-0022-543-H08C-8409
GCATTATAACCATTGAAATCAAATGATCAAGACGAAGCATATGATGGATGGTGCAGTGTT
CCCTTGAGGAAGGAAGAGTACCAGTGAGCAGAGAGTTTAGGGCTCCTCTTAAGAGAAGGA
TCCTTGAAGTCCACGTACAATAATCCAAAGCTCCTCTCGTACCCACCAAATAGCTCAAAC
ACATCCATCAACGACCACTGAAAATACCCTTTTACGTCCGATCCTTTCCTGCTGTGTCAA
ATTTTATGCTTAGAATCTGACTGACACATCCATTGAATGATAACTTGAACAACTTTTACT
GTTACCTGAGTGAATGAAGCACTGCTTTAATGTACGAGCTCAGATACTTCACCCTTGTTG
TGTCCACAAGTGATGAGCTGTGAGGAGTCATTTGTCCTGTAAATGAAGAATCAATCACCC
GACAGAGAGAGAGAGTTTAAGACACATGAAGAAGCAAACTAAAGTCTGAGAGAGGCAGAG
AGAGATAAAGAGAGACCATTCTCTAGGATATAGACAGGAGGGTTTCCATAAGTTTCTTTG
ACATACAAAAGTATCTGTTGCAGACTCCATGGTGTATTTGCATACTGAAAGAAGCAAATC
ATCAAACATCAGTCTTGTCTCTTGTAGGTGATCACATATAAAATTGATATCTACATACAA
ACCTCATTCTCGATTGATGTGTTTCCAACCACTGCAAAATGGAGAAACCAGTGAGCCTGC
AGATTTTTAACTTTTTATTCTCAGACAGTATCATATAACTTACAGGTCATTTCCACGGCT
ATATCTGTGTTGAAATCCTGG

Last Updated on 06.12.2018 16:11