Sequences from confirmation amplicon


Confirmation sequences for line 969B10 and hit At1g02900 are shown.
Line ID 969B10
Locus At1g02900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:654346
Primers used for PCR BU33 - 8474, show primer details
   
Sequence Name 35-K104354-0022-969-B10C-BU33
GenBank Accession LN501820 [GenBank]
Sequencing primer gene specific primer, BU33: 5'-AATTAAAATGAAAACGCTTGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104354-0022-969-B10C-BU33
GAAGATGCTTTCTTGTTTGTAGGTCTAACTCCTGCAACGAGCAATTTTGCTGCAACCACG
ACTATAAGGATTAGCCTGAGCTCCGTTCTGACAATTGTAATAAGACGCACCTCTTCTTGA
ACAAGGCACACTGTTCCGTTTCAAAGACTGATAGCTTATGTATTTTGTGGTCGCCAATAT
TCTTCTATTGATCTCTGAGTCCATTTCTTCTTCCTCCGCCCCGATACACTCTGCTATTGA
ACCGTGGCAGCCTGAACCATTGTCTCCAGTCGTAGCCCATGCTACACCACCGAGGTCGTT
GGCGAAGCCGGCTTGGACCGGAGGTGAAGAGATGATGAAGACGACGAGAATCGTAAGAGT
TAAGAACAGAGTAAAGGACTTGTCCATTTTCTTGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
   
Sequence Name 38-K104355-0022-969-B10C-8409
GenBank Accession LN502966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104355-0022-969-B10C-8409
TTACAATTGAATATATCCTGCAAGAAAATGGACAAGTCCTTTACTCTGTTCTTAACTCTT
ACGATTCTCGTCGTCTTCATCATCTCTTCACCTCCGGTCCAAGCCGGCTTCGCCAACGAC
CTCGGTGGTGTAGCATGGGCTACGACTGGAGACAATGGTTCAGGCTGCCACGGTTCAATA
GCAGAGTGTATCGGGGCGGAGGAAGAAGAAATGGACTCAGAGATCAATAGAAGAATATTG
GCGACCACAAAATACATAAGCTATCAGTCTTTGAAACGGAACAGTGTGCCTTGTTCAAGA
AGAGGTGCGTCTTATTACAATTGTCAGAACGGAGCTCAGGCTAATCCTTATAGTCGTGGT
TGCAGCAAAATTGCTCGTTGCAGGAGTTAGACCTACAAACAAGAAAGCTATCTTCTTGAT
CTTGACTTATGTTTTCTTTGATCCATCAAGCGTTTTCATTTTAA

Last Updated on 06.12.2018 16:11