Sequences from confirmation amplicon


Confirmation sequences for line 742D02 and hit At1g04220 are shown.
Line ID 742D02
Locus At1g04220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1120831
Primers used for PCR CR72 - 8474, show primer details
   
Sequence Name 76-K107287-0022-742-D02C-CR72
GenBank Accession LN513407 [GenBank]
Sequencing primer gene specific primer, CR72: 5'-GAGCTTCTCCAGCTATAGCCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K107287-0022-742-D02C-CR72
CGATTTGTTTGTTGCGTCGTTGTCTTCGCGTTGGTAACGCAGTTAAAGGCGTTGTCGTCA
GATCCTTTGTGTGTACGGACGGTGTGGATGAGTTGGTATTTGGAGCGGCAGCGGTCGGAG
GAGCGGTTAGAGAGGAGAACGGCGGCGCCACCCATTCGGAAGATGCAGTTTGAGAGAAGC
ATTGATCGGTCGTTGCCTAAATACCAGTTTAGGGTTATGTTCTCTGTGCTCACTACTAGT
GCATATGAATTTGGTTGAACCTGCATGCATATTACAATAAAAAAAAAAAAAACGGGCCCC
TTAACGCTTTTTTTTTAGCCCTTTTTACATTTCTTTACAAATTTGGGACTCTAAAAGCTT
TGGCTTTTTTTTATTTTTAAAAATTTGAAGACAAAGGATCCGGTTTGTAAATCGATGAAT
CCGGGATATTTTCATTTGAAAAGGGCTTCTGGCCGGGGAAATCTCCTGGGATCACCAAGG
AGTTTGAGGGCCAATTGGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAA
AATGTAAATGTCCCCCGCTTTTTTAAAAAAA
   
Sequence Name 60-K107286-0022-742-D02C-8409
GenBank Accession LN510277 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107286-0022-742-D02C-8409
TTACAATTGAATATATCCTGTATTCATCGATTTCATACAGTATCATCTGTCTTCATATTT
TTATAATTATATATATGCATATGCTTTTAGAGTCCTAATTTTGTAATGAATTGTAATATG
GACTAATAAATATGCGTTAAGGGTCCTGTTTTTTTTTTTTTTATTGAAAAATGCAGGCGG
GTTCAACCAAATTCATATGCCCTATTAGGGACCACAAAAAACAAACCCCTAAACGGGTTT
TTAGGCAACAACCAATAAAGGTTTCTCTAAAACTGCATTTTCCAAAGGGGGGGCCCCCCC
TTTCCCCTCTCAAACCGCCCCCCCAACCGCTGCCGCTCCAAATACCAACTCACCCACCCC
GCCCGTACACACAAGGGATTTGACAACACCCCCTTTAACGGGTTTTCCCACCGCGAAAAC
AACAACAACAACAAAAAAATGGGGGTCTCATTTTCCAAAAACCTAAGGGTTATGGCGGGA
AAACCAA

Last Updated on 06.12.2018 16:11