Sequences from confirmation amplicon


Confirmation sequences for line 188B10 and hit At1g05770 are shown.
Line ID 188B10
Locus At1g05770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1726111
Primers used for PCR KT21 - 8474, show primer details
   
Sequence Name 38-K100814-0022-188-B10C-8409
GenBank Accession LN502901 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K100814-0022-188-B10C-8409
GCATTTACAATTGAATATATCCTGAATAGAGAAAACACCGGTGGGACCATGAGATAGAAA
TATTTGAGAGATGATTCCATTGTGACTTCCTTCATCCCAATCGACCATTGTTTTCTTACC
TGAATAATCCGTTCCCACTGGTCCTATCTTGATTTTCCCTTCCATTTTCTTCTTTATGTG
TTTTTTTTGTTCCCTCTTACTTTAAGGATAATGCAAGAGTTAATTTCTTATAAGTCGGTG
TTTTTAGGATAATCTTGTTACATGATGAGATATTCCAATCTTGCATAACTTGTAAACAAT
ATATATTCCTTACCTTTATTCCATGTCTATCTATTTAATAAGTCATAAATTGAAACATAT
CAAATAATCAAACCGTCAAAATTAATTGTAGTACAAAAAAAACCGTTAAAATTAAACAAA
AACCGTCAAAATAATCTTAGTAAAAAAATACCGTCAAGATTAATGTTTGTACCGTCCCAC
GAATCGGCTTATACCGCTCTTACAAGCAATCTTATTTAACCCGCCGAGTCTAAGACCGAG
AGTGCACTCGCTAGATCCAACGCCGGACTCAATCCATCCCTTAACCCTGAGCAAGATCTG
TGTCTC
   
Sequence Name 38-K100826-0022-188-B10C-KT21
GenBank Accession LN502903 [GenBank]
Sequencing primer gene specific primer, KT21: 5'-AATCAGGAATCGAGAGACACAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K100826-0022-188-B10C-KT21
GATCTAGCGAGTGCACTCTCGGTCTTAGACTCGGCGGGTTAAATAAGATTGCTTGTAAGA
GCGGTATAAGCCGATTCGTGGGACGGTACAAACATTAATCTTGACGGTATTTTTTTACTA
AGATTATTTTGACGGTTTTTGTTTAATTTTAACGGTTTTTTTTGTACTACAATTAATTTT
GACGGTTTGATTATTTGATATGTTTCAATTTATGACTTATTAAATAGATAGACATGGAAT
AAAGGTAAGGAATATATATTGTTTACAAGTTATGCAAGATTGGAATATCTCATCATGTAA
CAAGATTATCCTAAAAACACCGACTTATAAGAAATTAACTCTTGCATTATCCTTAAAGTA
AGAGGGAACAAAAAAAACACATAAAGAAGAAAATGGAAGGGAAAATCAAGATAGGACCAG
TGGGAACGGATTATTCAGGTAAGAAAACAATGGTCGATTGGGATGAAGGAAGTCACAATG
GAATCATCTCTCAAATATTTCTATCTCATGGTCCCACCGGTGTTTTCTCTATTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGTATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11