Sequences from confirmation amplicon


Confirmation sequences for line 819C12 and hit At1g05785 are shown.
Line ID 819C12
Locus At1g05785 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1730357
Primers used for PCR 32oh - 8474, show primer details
   
Sequence Name 45-K114805-0022-819-C12C-32oh
GenBank Accession LN505626 [GenBank]
Sequencing primer gene specific primer, 32oh: 5'-ACACCACACATGAACTCAACAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114805-0022-819-C12C-32oh
CAGCTTCAATTTTTTCTTCCTTTTCGAGAAGATTGATCCTAAAATATAATTACCCTAAAC
CCTAGAAAAGTTATATGCCTTGTGGGTTTTCATCTCTTAAAACAGTAAACTGATGTCAAC
CCAAGTTAAGCTCAAGAAGACCAAAGATCATCCGCACCCAATCAACGATCAATTTTACAA
TCTTTTTACTTTTTTTTTTTAATTGAAAAAATGGCATTCCTCCAAAACAAATTTTTTAAT
TTCAAATCTCAATTTCAAAACAAAATTGGAAATAATAAATTGTAATTGGTTTCTTTTCCG
GAAATTCTCCTGGGATAACCATTGATTATAAGGGTAAATATGGAAAAAAAAAAAAATTAT
TTCCCATTTTTTTTTCATTTCAAAATTGTAAATTTCC
   
Sequence Name 21-K114804-0022-819-C12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114804-0022-819-C12C-8409
TCGAATTCGAGAATTCCGAACTCCAAAGAATTTTCGTTTTCGGAGGATTCGCAATTCTTT
TCAATCAAAAAAAAAAATTAAAAAAATTGTAAATTTGATCTTTAATGGGGTGCGAATAAT
CTTT

Last Updated on 06.12.2018 16:11