Sequences from confirmation amplicon


Confirmation sequences for line 290H10 and hit At1g06190 are shown.
Line ID 290H10
Locus At1g06190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1894097
Primers used for PCR 17ss - 8474, show primer details
   
Sequence Name 49-K116478-0022-290-H10C-17ss
GenBank Accession LN506956 [GenBank]
Sequencing primer gene specific primer, 17ss: 5'-TATTCATAGAACCCTAGAGGCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116478-0022-290-H10C-17ss
ACGAATAGCAGAAGTCTATAAAACTCAAGAGAATCATTTCATCGAACAACTGGNTGGACA
ACAAACCAAAAGCAAAATGATTTCTCCACACACATGCCGTTTCCGCCTACAGTGACAGGG
GCATAAAACACTAGGTGCTCGGCGAGAATTCAAGGAAGAACAGTAACAATATTCCCGGAA
AACAAATAGAGACGCACATAGATAGATGCCTTCACAATTATCAAAATAAAACAGAAAAAT
TATCGACAAGCGAGAAAGAGAGAGACATGAAAAGGGTACAGGATAGCGAAGTACCAGGGA
CATAGTCAGAAGTCAAATGGAAAGTTCCCGACATCGCCATGAACAGACTTCAGGTGCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGATTATTAT
   
Sequence Name 24-K116477-0022-290-H10C-8409
GenBank Accession LN497143 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116477-0022-290-H10C-8409
ATTTCAATTGAATATATCCTGCACCTGAAGTCTGTTCATGGCGATGTCGGGAACTTTCCA
TTTGACTTCTGACTATGTCCCTGGTACTTCGCTATCCTGTACCCTTTTCATGTCTCTCTC
TTTCTCGCTTGTCGATAATTTTTCTGTTTTATTTTGATAATTGTGAAGGCATCTATCTAT
GTGCGTCTCTATTTGTTTTCCGGGAATATTGTTACTGTTCTTCCTTGAATTCTCGCCGAG
CACCTAGTGTTTTATGCCCCTGTCACTGTAGGCGGAAACGGCATGTGTGTGGAGAAATCA
TTTTGCTTTTGGTTTGTTGTCCACCAGTTGTTCGATGAAATGATTCTCTTGAGTTTTATA
GACTTCTGCTTATTCGTGTGTGTAATGCAACTTGCTTCTGTACTGGCCTGCCTCTAGGGT
CTTATGAATAA

Last Updated on 06.12.2018 16:11