Sequences from confirmation amplicon


Confirmation sequences for line 013F09 and hit At1g06400 are shown.
Line ID 013F09
Locus At1g06400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1951673
Primers used for PCR 01eq - 8474, show primer details
   
Sequence Name 51-K111269-0022-013-F09C-01eq
GenBank Accession LN507539 [GenBank]
Sequencing primer gene specific primer, 01eq: 5'-CTGAAGTCTCCATGAAATAGAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111269-0022-013-F09C-01eq
CAAGTGACGCAGATCGCACTTGTTCCCTATAAGCATCACCACTATGTTTGGGTCTGTGTG
TCCTCTTAGTTCCCTTAGCCATCTTGCTGCATTCTCAAATGTTGCGTGCCTTGTCACGTC
GTAAATAAGCAAAGCACCAACAGCTCCTCTGTAATAAGCGCTTGTGATTGCTCGGTATCT
ATCATAGCATAAACAAAACCAGCATTTTCTCAGATGTTGTTAACAAAGATATAATAACAA
CTACAATAATAAGTTCAGTAACATTACAGATTAAGTACACAAAGTTAGACTATTTTCGAT
AACCATATTACATGGTATCCATCTACCAAATGGCTTTAATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATT
ATTATATCTATGTTNGCNNCCTCCGTGAGAGTGCTGGTTGGGTTTGTGCTTGGATAGATA
CAGAGCGATCACAAGCGCTTATTACAGAGGAGCTGTTGGTGGGTGGCTTATTTACGACGT
GACTAGGCACGCACCATGTGTGAATGCAGGAAGATGGCTGAGGGAGCTAAGAGGACACGC
AGACCCAAACATAGTGGTGATGCTTATAGGGAACAAGTGCGATCTGCGTCACTTGGTGGC
AGTCAAAACAGAAGAGGCTAAAGCCTTTGCTGAGAGAGAATCCCTCTATTTC
   
Sequence Name 35-K111268-0022-013-F09C-8409
GenBank Accession LN501874 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111268-0022-013-F09C-8409
TCATTGAATATATCCTGATTAAAGCCATTTGGTAGATGGATACCATGTAATATGGTTATC
GAAAATAGTCTAACTTTGTGTACTTAATCTGTAATGTTACTGAACTTATTATTGTAGTTG
TTATTATATCTTTGTTAACAACATCTGAGAAAATGCTGGTTTTGTTTATGCTATGATAGA
TACCGAGCAATCACAAGCGCTTATTACAGAGGAGCTGTTGGTGCTTTGCTTATTTACGAC
GTGACAAGGCACGCAACATTTGAGAATGCAGCAAGATGGCTAAGGGAACTAAGAGGACAC
ACAGACCCAAACATAGTGGTGATGCTTATAGGGAACAAGTGCGATCTGCGTCACTTGGTG
GCAGTCAAAACAGAAGAGGCTAAAGCCTTTGCTGAGAGAGAATCCCTCTATTTCATGAGA
ACTTCA

Last Updated on 06.12.2018 16:11