Sequences from confirmation amplicon


Confirmation sequences for line 561H09 and hit At1g06950 are shown.
Line ID 561H09
Locus At1g06950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2134362
Primers used for PCR 14ey - 8474, show primer details
   
Sequence Name 83-K111271-0022-561-H09C-14ey
GenBank Accession LN514328 [GenBank]
Sequencing primer gene specific primer, 14ey: 5'-CAATGTTAAATGAGAAGGGACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K111271-0022-561-H09C-14ey
TTAGGTGGTATTCAGACAACTTGCTGATAAACGAATATTACAAAACCCAATGGACAAAGA
AGTGCCACAATAATCAATATAACCAAGTCAAGCAAACTCAAACCCGTTCTACAAATGTAA
CATTACTTCCCATCTCAAAATGTAAAACATGAACAACATTACCTCTACCAACTAATTTTA
ACCGTTCGGCATACAGCTGCTTTGCATTTTCACGAATAGCAATCTCAACCTAGAAATGAG
GTCAAGACATTAGCACAGAACTTCAGATTACCAAGAAGGAGTGAGTTGCCAAAATGAAAC
TTCCATAAAACAACTATTGCGCAAAATCAGAAGTATGAAAAAAATTGGAAATGCCTACCT
GAGCATCTGTGACCTTCAATACTCGCTTCCAAGGTAGAAGGCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 68-K111270-0022-561-H09C-8409
GenBank Accession LN512041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K111270-0022-561-H09C-8409
TCGGAAGCCTTCTACCTTGGAGCGAGTATTGAAGGTCACAGATGCTCAGGTAGGCATTTC
CAATTTTTTTCATACTTCTGATTTTGCGCAATAGTTGTTTTATGGAAGTTTCATTTTGGC
AACTCACTCCTTCTTGGTAATCTGAAGTTCTGTGCTAATGTCTTGACCTCATTTCTAGGT
TGAGATTGCTATTCGTGAAAATGCAAAGCAGCTGTATGCCGAACGGTTAAAATTAGTTGG
TAGAGGTAATGTTGTTCATGTTTTACATTTTGAGATGGGAAGTAATGTTACATTTGTAGA
ACGGGTTTGAGTTTGCTTGACTTGGTTATATTGATTATTGTGGCACTTCTTTGTCCATTG
GGTTTTGTAATATTCGTTTATCAGCAAGTTGTCTGAATACCAACCTTAATTGTTACTTCG
CATGCGACTGTAATGGTCCCTTCTCATNTAACATT

Last Updated on 06.12.2018 16:11