Sequences from confirmation amplicon


Confirmation sequences for line 002C02 and hit At1g07720 are shown.
Line ID 002C02
Locus At1g07720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2392686
Primers used for PCR BJ01 - 8474, show primer details
   
Sequence Name 42-K104374-0022-002-C02C-BJ01
GenBank Accession LN504451 [GenBank]
Sequencing primer gene specific primer, BJ01: 5'-CGAAGGTGAAATTTTGTTTCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104374-0022-002-C02C-BJ01
ACTCTTCCATCTCCGAGAGCCCATCCTGTAGCGTAGGACGCTGTTCACGGCCCTCAAAGA
AGAGCCTTGGTGCGTAAGTTTGCTCTCCGATACCGGAACTAACGATGGCTTTGAGGAGGA
ACTTGTACTCGTTGAGCCGAAGGTGTTTGTTTCGAAGGATGATATCACCGCTGAATTGAG
TGTTCACCATACGATCGTCGGAAGGTTTGTGACATTGGTAGTCCAAGATGTAGCAGTTTT
GGTCTCTCTTGGAGTCTATGCGTTTCCAGATCTTGAAGATGAGATAAGAGACGAGAAGAG
AGAGGAGCATAACAAGAAGATCCATTATTGTGTTTTTATTGGAGTTAGAAAGAGGAGTGA
TTAAAAATGCATGAAAATGTAGCAAAGATTGTTGTGGGATATATAAGAGGACAGTGGAGC
AGGAAAAGACAGCTCTCGTTTTTTGACAACTCATTCCGTCCAATAGATTATGTCAATATC
ATTTAGTAAATTCTCGGGGGTTAATAAAAGACAGACTTGTATTTAATTGGAAAAGTTACA
TGCAAGGACAAAGTTATGGTAAGAAAAATTGAAACAATAAGATGGCACATTGATGATTGA
TGCTATGATGTGATATCAGGATAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 58-K104375-0022-002-C02C-8409
GenBank Accession LN509697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K104375-0022-002-C02C-8409
ATTTACATTGAATATATCCTGATTATCCTGATATCACATCATAGCATCAATCATCAATGT
GCCATCTTATTGTTTCAATTTTTCTTACCATAACTTTGTCCTTGCATGTAACTTTTCCAA
TTAAATACAAGTCTGTCTTTTATTAACCCCCGAGAATTTACTAAATGATATTGACATAAT
CTATTGGACGGAATGAGTTGTCAAAAAACGAGAGCTGTCTTTTCCTGCTCCACTGTCCTC
TTATATATCCCACAACAATCTTTGCTACATTTTCATGCATTTTTAATCACTCCTCTTTCT
AACTCCAATAAAAACACAATAATGGATCTTCTTGTTATGCTCCTCTCTCTTCTCGTCTCT
TATCTCATCTTCAAGATCTGGAAACGCATAGACTCCAAGAGAGACCAAAACTGCTACATC
TTGGACTACCAATGTCACAAACCTTCCGACGATCGTATGGTGAACACTCAATTCAGCGGT
GATATCATCCTTCGAAACAAACACCTTCGGCTCAACGAGTACAAGTTCCTCCTCAAAGCC
ATCGTTAGTTCCGGTATCGGAGAGCAAACTTACGCACCAAGGCTCTTCTTTGAGGGCCGT
GAACAGCGTCCTACGCTACAGGATGGGCTCTCGGAGATGGAAGAGTTTTACATAGACACC
ATCGAGAAAGTCCTTAAGAGAAACA

Last Updated on 06.12.2018 16:11