Sequences from confirmation amplicon


Confirmation sequences for line 513G04 and hit At1g07930 are shown.
Line ID 513G04
Locus At1g07930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2459606
Primers used for PCR IW58 - 8474, show primer details
   
Sequence Name 33-K033213-022-513-G04C-8409
GenBank Accession LN500895 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033213-022-513-G04C-8409
CCTTCTTCATTGAATATATCCTGGCCTTGGAGTACTTGGGGGTAGTGGCATCCATCTACA
AACAACATGAAAGAAAAATCCAATTAATGAGACTGAACACAAACAATTTGAAGCAATAAT
CACATAAGAGATAAAAGAAGCTTTTGTTACCTTGTTACAACAGCAGATCATCTGCTTGAC
ACCAAGGGTGAAAGCAAGGAGAGCGTGCTCACGGGTCTGACCATCCTTGGAGATACCAGC
CTCAAAACCACCAGTGGTGGAGTCAATGATAAGGACAGCACAATCAGCCTGGGAGGTACC
AGTGATCATGTTCTTGATGAAATCACGATGACCAGGAGCATCAATGACAGTGCAGTAGTA
CTTGGTGGTCTCGAACTTCCAGAGAGCAATGTCAATGGTGATACCACGCTCACGCTCAGC
CTTAAGTTTGTCCAACACCCATGCGTACTTGAAGGACCTCTTGTTCATCTCAGCAGCCTC
CTTCTCGAACCTCTCGATCACACGCTTGTCAATACCACCCAACTTGTAGATCAAGTGTCC
AGTGGTTGTCGACTTTCCAGAATCGACGTGGCCAATGACCACAATGTTGATGTGAAACTT
CTCTTTACCCATGGTTAGCTACTTTTCAGACAAATCTGCAAAAAAAAGAACAATCAAATA
TTGAATCAATAAAAACAGTAGACATATGAACACAAAAAACAATCATTGTTAAAAGCCGCT
ATCGGATTCAAACCGATTTCTACCACAAAATTGTGGTGCTCAATTCATC
   
Sequence Name 41-K033213-022-513-G04C-IW58
GenBank Accession LN503981 [GenBank]
Sequencing primer gene specific primer, IW58: 5'-TCAGATGATAGAGCACCACAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K033213-022-513-G04C-IW58
TCGATAGCGGCTTTTACAATGATTGTTTTTTGTGTTCATATGTCTACTGTTTTTATTGAT
TCAATATTTGATTGTTCTTTTTTTTGCAGATTTGTCTGAAAAGTAGCTAACCATGGGTAA
AGAGAAGTTTCACATCAACATTGTGGTCATTGGCCACGTCGATTCTGGAAAGTCGACAAC
CACTGGACACTTGATCTACAAGTTGGGTGGTATTGACAAGCGTGTGATCGAGAGGTTCGA
GAAGGAGGCTGCTGAGATGAACAAGAGGTCCTTCAAGTACGCATGGGTGTTGGACAAACT
TAAGGCTGAGCGTGAGCGTGGTATCACCATTGACATTGCTCTCTGGAAGTTCGAGACCAC
CAAGTACTACTGCACTGTCATTGATGCTCCTGGTCATCGTGATTTCATCAAGAACATGAT
CACTGGTACCTCCCAGGCTGATTGTGCTGTCCTTATCATTGACTCCACCACTGGTGGTTT
TGAGGCTGGTATCTCCAAGGATGGTCAGACCCGTGAGCACGCTCTCCTTGCTTTCACCCT
TGGTGTCAAGCAGATGATCTGCTGTTGTAACAAGGTAACAAAAGCTTCTTTTATCTCTTA
TGTGATTATTGCTTCAAATTGTTTGTGTTCAGTCTCATTAATTGGATTTTTCTTTCATGT
TGTTTGTAGATGGATGCCACTACCCCCAAGTACTCCAAGGCCAGGATATATTCAATTGAA
GAAGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATATTCTAATTCGGAAATCTTA

Last Updated on 06.12.2018 16:11