Sequences from confirmation amplicon


Confirmation sequences for line 271E01 and hit At1g08070 are shown.
Line ID 271E01
Locus At1g08070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2514830
Primers used for PCR 41pv - 8474, show primer details
   
Sequence Name 38-K115234-0022-271-E01C-41pv
GenBank Accession LN503093 [GenBank]
Sequencing primer gene specific primer, 41pv: 5'-GATGATATCACGTTTGTTGGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115234-0022-271-E01C-41pv
ATCTTGGACGCCATATTTTCAGAACATGACACAGGACTACAAAATGACCCCAAAATTAGA
GCACTACGGTTGCATGATTGATCTTTTGGGTCATTCGGGTCTATTCAAAGAAGCGGAAGA
AATGATAAACATGATGGAAATGGAGCCCGATGGAGTGATTTGGTGCTCCCTTCTCAAAGC
TTGCAAGATGCATGGGAATGTCGAGTTGGGCGAATCATTTGCAGAAAACCTCATTAAGAT
TGAGCCAGAAAACCCAGGTTCTTATGTACTTCTGTCTAACATTTATGCCTCAGCAGGAAG
ATGGAACGAAGTAGCAAAAACACGACAAATTTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTAACAATTCAAAAATGTAGATGTC
CGC
   
Sequence Name 14-K115233-0022-271-E01C-8409
GenBank Accession LN492635 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115233-0022-271-E01C-8409
TTCGTTCATCTTCCTGCTGAGGCATAAATGTTAGACAGAAGTACATAAGAACCTGGGGTT
TTCTGGCTCAATCTTAATGAGGTTTTCTGCAAATGATTCGCCCAACTCGACATTCCCATG
CATCTTGCAAGCTTTGAGAAGGGAGCACCAAATCACTCCATCGGGCTCCATTTCCATCAT
GTTTATCATTTCTTCCGCTTCTTTGAATAGACCCGAATGACCCAAAAGATCAATCATGCA
ACCGTAGTGCTCTAATTTTGGGGTCATTTTGTAGTCCTGTGTCATTGTTCTGAAAATATG
GCGTCCAAGATCTAGCATACCTGAATGGCTACAAGCAGACAACAAACCAACAAACTGNAT
ATCAT

Last Updated on 06.12.2018 16:11