Sequences from confirmation amplicon


Confirmation sequences for line 775E07 and hit At1g08090 are shown.
Line ID 775E07
Locus At1g08090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2524512
Primers used for PCR 48sa - 8474, show primer details
   
Sequence Name 66-K116229-0022-775-E07C-48sa
GenBank Accession LN511688 [GenBank]
Sequencing primer gene specific primer, 48sa: 5'-GACCTAGATTGAGCACCAAGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K116229-0022-775-E07C-48sa
CGATCCTCCAGGCCGTGTAGGCTGTGGAACCGCAGCGCCTAACGATTTCATACACAATGG
GCATGAGCAACTGCGTTATGCCGCCACCCATGTTTCCCCATCCGGCTGCTGTCCCATTCA
CCAGACCAATGATCTGACTGTTGAACATAGTGCTCATCCAGTATTGACAAGAACCCAAAC
GTCGCCAGGCAAAAACCAATCATGAACCTCACCGTTATGAAGCCTGCTGCGTCACTCACG
AAGCTCATGGAGAACACCGTTGGGGCAGACAACATCACAAGGAAGGCACAACCGTAACGG
GGACCCAAAAGATCACACACGGCTCCCATCACGAGCCTAGGATATATTCAATTGTAAATG
GCTTCATGTCCGTTAAATCTACATGGATCAGCAATGACTATGATGGTCAATATGGACAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTC
   
Sequence Name 26-K116228-0022-775-E07C-8409
GenBank Accession LN498077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116228-0022-775-E07C-8409
TGGGTTCCCGTTACGGTTGTGCCTTTCCTTGTGATGTTGTCTGCCCCAACGGTGTTCTCC
ATGAGCTTCGTGAGTGACGCAGCAGGCTTCATAACGGTGAGGTTCATGATTGGTTTTTGC
CTGGCGAACGTTTGTGTCTTGTCAATACTGGATGAGCACTATGTTCAACAGTCAGATCAT
TGGTCTGGTGAATGGGACAGCAGCCGGATGGGGAAACATGGGTGGCGGCATAACGCAGTT
GCTCATGCCCATTGTGTATGAAATCATTAGGCGCTGCGGTTCCACAGCCTTCACGGCCTG
GAGGATCGCCTTCTTTGTACCCGGTTGGTTGCACATCATCATGGGAATCTTGGTGC

Last Updated on 06.12.2018 16:11