Sequences from confirmation amplicon


Confirmation sequences for line 840D12 and hit At1g08430 are shown.
Line ID 840D12
Locus At1g08430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2659274
Primers used for PCR 53sa - 8474, show primer details
   
Sequence Name 58-K116321-0022-840-D12C-53sa
GenBank Accession LN509802 [GenBank]
Sequencing primer gene specific primer, 53sa: 5'-AATTATTCATGCTTTCAAAGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116321-0022-840-D12C-53sa
CCTTTCGGTCCTTTCCCGATTACTTCGGTATCAATGCAATGTGGGCTGTCATGACCGTCG
TTGTTGTCTTTGAATTCTCCGTAGGTTCGTCATCTAGTTTCTATCATTTGAATATGTCTA
GTAGATATATCTAGGGAGAATGCAAGAATTTTTGTTGTAAACTTTTGGCCATAATATTAA
ATAACTTTGGTTTGATGTGTACTTAGGGGCTACACTTGGGAAAGGACTAAATAGAGGAGT
GGCAACATTAGTAGCAGGAGGACTAGGAATTGGAGCTCATCAACTAGCAAGATTGTCGGG
TGCAACAGTGGAACCTATTCTTTTGGTCATGTTGGTCTTTGTGCAAGGAAAAGATCATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGGTTTATTAT
   
Sequence Name 26-K116320-0022-840-D12C-8409
GenBank Accession LN498081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116320-0022-840-D12C-8409
ATTTCAATTGATATGATCTTTTCCTTGCACAAAGACCAACATGACCAAAAGAATAGGTTC
CACTGTTGCACCCGACAATCTTGCTAGTTGATGAGCTCCAATTCCTAGTCCTCCTGCTAC
TAATGTTGCCACTCCTCTATTTAGTCCTTTCCCAAGTGTAGCCCCTAAGTACACATCAAA
CCAAAGTTATTTAATATTATGGCCAAAAGTTTACAACAAAAATTCTTGCATTCTCCCTAG
ATATATCTACTAGACATATTCAAATGATAGAAACTAGATGACGAACCTACGGAGAATTCA
AAGACAACAACGACGGTCATGACAGCCCACATTGCATTGATACCGAAGTAATCGGTGAAA
GGACCGAAAGGTTGGTAATAATAGAAGGAAGAGACCAAAACAAGAGCAAGTCCCACTTTG
AAAGCAGAAATAAT

Last Updated on 06.12.2018 16:11