Sequences from confirmation amplicon


Confirmation sequences for line 097F11 and hit At1g08880 are shown.
Line ID 097F11
Locus At1g08880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2848065
Primers used for PCR TA18 - 8474, show primer details
   
Sequence Name 10-K030139-022-097-F11C-8409
GenBank Accession LN490525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K030139-022-097-F11C-8409
AACCACAATAAATGTATTGATGTAAATTAAAAATATGAACTTGTAACAGATCAAGATTCA
GGGTCTAAAATTATAGAAAGCTTAATAATGGAGGACTATTTCACTAAAATCACTTTTCGT
TTGTACATTATTTTCAAAAAGTAAAAGGAGATGTACGAACCATAGATCACATAATAATTG
AAAGGGTAGATGATCTGCCACGTTGGCAATCCGTGTGATCTAAAGTCTAACAAATCACAA
TCAATCTTAGTAGCCTATACATATAGAGAAGAGCAAAACCCTAAAGCCCACTCATCTTCT
CAA
   
Sequence Name 10-K030139-022-097-F11C-TA18
GenBank Accession LN490526 [GenBank]
Sequencing primer gene specific primer, TA18: 5'-AATTGAGAAGATGAGTGGGCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K030139-022-097-F11C-TA18
AGATTGATTGTGATTTGTTAGACTTTAGATCACACNGGATTGCCAACGTGGCAGATCATC
TACCCTTTCAATTATTATGTGATCTATGGTTCGTACATCTCCTTTTACTTTTTGAAAATA
ATGTACAAACGAAAAGTGATTTTAGTGAAATAGTCCTCCATTATTAAGCTTTCTATAATT
TTAGACCCTGAATCTTGATCTGTTACAAGTTCATATTTTTAATTTACATCAATACATTTA
TTGTGGTTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CACG

Last Updated on 06.12.2018 16:11