Sequences from confirmation amplicon


Confirmation sequences for line 027A06 and hit At1g09460 are shown.
Line ID 027A06
Locus At1g09460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3054301
Primers used for PCR DC70 - 8474, show primer details
   
Sequence Name 55-K032605-022-027-A06C-8409
GenBank Accession LN508714 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K032605-022-027-A06C-8409
ATTGAATATATCCTGAGACCGATGGAGAGTTTGAGACAACAGGCGGTGCAACCACAGGAA
CAGGTACTGGGACCGTCCCTGAGGGTTGTGTTGGAGGATACTGAGTCACTGGGTTGGTTA
CTGGGTTTGTCACAGGTGGTGTGAGTGTAGGAGGAGGAATTGTTATGACTGGAGGTGGTG
TGAGTGTAGGAGGTGGAATAGTTGTGACTGGAGGAACAATAGGAACTGTTGTGGCTGGAT
TTGTTGGCGTTATGGTCACCGGAGGAAATGTAGCCGTCGGTGTAGTTGTGGGGTTTGTAG
GAAAGACCGTTGGTGGGTTTAACACATCATGATGTGTAGTTGTTGAAGAACAGTATGAGA
GAAGAGAAAGAAACAAGAAGGTGAAGACGAAGAAGTAACTCTGTTTCGCCATCACAGCTT
GGGATTGGTTTTGTTTTTCTTCTTCTTCTTTCTTGGGTTTATTATAAGTACATGATTTGT
AAGAAGAACAAAAGCTTAGAGATTCTCTTGAGAGAATTGGGGCAGAAAAAGAA
   
Sequence Name 55-K032605-022-027-A06C-DC70
GenBank Accession LN508715 [GenBank]
Sequencing primer gene specific primer, DC70: 5'-TCAGCATCTTCTCTCTTTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K032605-022-027-A06C-DC70
CTAGCTTTTGTTCTTCTTACAAATCATGTACTTATAATAAACCCAAGAAAGAAGAAGAAG
AAAAACAAAACCAATCCCAAGCTGTGATGGCGAAACAGAGTTACTTCTTCGTCTTCACCT
TCTTGTTTCTTTCTCTTCTCTCATACTGTTCTTCAACAACTACACATCATGATGTGTTAA
ACCCACCAACGGTCTTTCCTACAAACCCCACAACTACACCGACGGCTACATTTCCTCCGG
TGACCATAACGCCAACAAATCCAGCCACAACAGTTCCTATTGTTCCTCCAGTCACAACTA
TTCCACCTCCTACACTCACACCACCTCCAGTCATAACAATTCCTCCTCCTACACTCACAC
CACCTGTGACAAACCCAGTAACCAACCCAGTGACTCAGTATCCTCCAACACAACCCTCAG
GGACGGTCCCAGTACCTGTTCCTGTGGTTGCACCGCCTGTTGTCTCAAACTCTCCATCGG
TCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTAC
Primers used for PCR FP25 - 3144, show primer details
   
Sequence Name 33-K032925-022-027-A06C-3144
GenBank Accession LN500891 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K032925-022-027-A06C-3144
TCCACTACCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCACAGGCACTTGACTATGCC
TGCGGGATCGCGGATTGCTCTCAACTACAGCAAGGCGGCAATTGTTATTCTCCTATCAGT
CTTCAGAGTCATGCCTCATTTGCCTTCAACAGCTATTACCAGAAGAATCCTTCTCCACAA
AGCTGTGATTTTGGCGGTGCAGCCTCATTAGTTAACACCAACCCAAGTGAGTGAGAAAAA
ACAGAAGATACTCTGTTTCTCTTTTTCTTTCTTGTTTTCAGTTAGATGGCTTTGATTGAC
TTTGGNTTTGGAT
   
Sequence Name 33-K032925-022-027-A06C-FP25
GenBank Accession LN500892 [GenBank]
Sequencing primer gene specific primer, FP25: 5'-AATCCAAACCAAAGTCAATCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K032925-022-027-A06C-FP25
GAAGAAAGAGAAACGAGTATCTTCTGTTTTTTCTCACTCACTTGGGTTGGTGTTAACTAA
TGAGGCTGCACCGCCAAAATCACAGCTTTGTGGAGAAGGATTCTTCTGGTAATAGCTGTT
GAAGGCAAATGAGGCATGACTCTGAAGACTGATAGGAGAATAACAATTGCCGCCTTGCTG
TAGTTGAGAGCAATCCGCGATCCCGCAGGCATAGTCAAGTGCCTGTTGAACTTCCTTATA
TAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGA
TATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGC
TCCTCGTGGGTGGGGGTCCCATCTTTGG

Last Updated on 06.12.2018 16:11