Sequences from confirmation amplicon


Confirmation sequences for line 483H11 and hit At1g09570 are shown.
Line ID 483H11
Locus At1g09570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3099853
Primers used for PCR 55ys - 8474, show primer details
   
Sequence Name 55-K122490-0022-483-H11C-55ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 55ys: 5'-AGAGGACTGAGTCGGCCTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K122490-0022-483-H11C-55ys
ACACAAAAAATAAGTGCAAAACTCTGATAAAAGAGGGAATAAAACATTCATGCCATAATC
ATATGAACACAAAAACATTCATCACCAGTCATACAAGCGAATGCATAAGCATAAACTTGA
AACACTAAGAACCAATTCAGAATTTCAAACACACAGTAAAAAGAGTTATGAACTTGACTA
ACTCCATCGAGAATACATAGCTAAACCCAGAAACAGGACGCAACAGATGCAGGAATCATC
GTGTCATATTCCAAGGTTCAATGATTTATCATTTTCAAAGTAACTGAACCAGTCAGATTC
CATGAACACTTCCTTTCTAAGTCATCCTACAATCCAAAATCAACAACATCTTGACTATGA
ACAAGTCGCACAAATAATAGTTGATAGTTAACAGGGTAAATCCACTACACAAATACTAAC
AAAACGAGGATCTGAGCTAATATCAGCCTCTCAAGATTCAAACACAGCTACATACATAAA
GTAGACTCAATAATAAACTCTTAAGAGTCTTTATAGCTAGCTATACCTTTCAAAAGGAAC
AATAAAGTCCCCACAGGGAAAGCAATCGTAGGAGTTAAAAGCGATAAAAGGATGAGATTA
ATTAATTGGATGCAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 23-K122489-0022-483-H11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K122489-0022-483-H11C-8409
GAATATATACTCATTGCATCCAATTAATTAATCTCATCCTTTTATCGCTTTTAACTCCTA
CGATTGCTTTCCCTGTGGGGACTTTATTGTTCCTTTTGAAAGGTATAGCTAGCTATAAAG
ACTCTTAAGAGTTTATTATTGAGTCTACTTTATGTATGTAGCTGTGTTTGAATCTTGAGA
GGCTGATATTAGCTCAGATCCTCGTTTTGTTAGTATTTGTGTAGTGGATTTACCCTGTTA
ACTATCAACTATTATTTGTGCGACTTGTTCATAGTCAAGATGTTGTTGATTTTGGATTGT
AGGATGACTTAGAAAGGAAGTGTTCATGGAATCTGACTGGTTCAGTTACTTTGAAAATGA
TAAATCATTGAACCTTGGAATATGACACGATGATTCCTGCATCTGTTGCGTCCTGTTTCT
GGGTTTAGCTATGTATTCTCGATGGAGTTAGTCAAGTTCATAACTCTTTTTACTGTGTGT
TTGAAATTCTGAATTGGTTCTTAGTGTTTCAAGTTTATGCTTATGCATTCGCTTGTATGA
CTGGTGATGAATGTTTTTGTGTTCATATGATTATGGCATGAATGTTTTATTCCCTCTTTT
ATCAGAGTTTTGCACTTATTTTTTGTTGTCTTGTCTCTGTGTCAGGAAAAAAATGTCAGG
CTCTAGGCCG

Last Updated on 06.12.2018 16:11