Sequences from confirmation amplicon


Confirmation sequences for line 170F02 and hit At1g10130 are shown.
Line ID 170F02
Locus At1g10130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3319325
Primers used for PCR MS02 - 8474, show primer details
   
Sequence Name 54-K016396-022-170-F02C-8409
GenBank Accession LN508320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K016396-022-170-F02C-8409
TCTCTTCCTCTCGTTCAGACATGGTAATCAGTTGACATGGTGATCCAATTATGAGGTCCT
TCCGCGGTCGTTTTCTTTTATCCCTAATGTTGCAATTGAAAACAAAGCATATAGTGAAAA
AACAAATCTGGTTATTGCTTGTGGTATAAAAATGATACAGTGTAATGTGACTGCTATACA
GAATTCTTCAGAGCTAGCACACTAGTTATGCTACTAAGACTGGACTGTTACTGTTCCTTC
CTTCAATAAACTGGCTTGCTAAGGTTTTTTATCTCTATGCTCTTCTGTGTTTAACCTATT
TTTCTCTCCTTCCTCTGAATTCCAGAGTGCTTCAGATATGGTTTTGGCTGATGATAATTT
TGCTTCAATAGTTGCGGTATGTTTGCTCCTTCCATTTTTCTTATGGTTTTTCCTTAATGC
TTGATTCTGGCATACAGTTGGCA
   
Sequence Name 55-K016396-022-170-F02C-MS02
GenBank Accession LN508647 [GenBank]
Sequencing primer gene specific primer, MS02: 5'-CATATGAATTTGCCAACTGTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K016396-022-170-F02C-ms02
GCATTAGGAAAAACCATAAGAAAAATGGAAGGAGCAAACATACCGCAACTATTGAAGCAA
AATTATCATCAGCCAAAACCATATCTGAAGCACTCTGGAATTCAGAGGAAGGAGAGAAAA
ATAGGTTAAACACAGAAGAGCATAGAGATAAAAAACCTTAGCAAGCCAGTTTATTGAAGG
AAGGAACAGTAACAGTCCAGTCTTAGTAGCATAACTAGTGTGCTAGCTCTGAAGAATTCT
GTATAGCAGTCACATTACACTGTATCACTTCTATACCACAAGCAATAACCAGATTTGTTT
TTTCACTATATGCTTTGTTTTCAATTGCAACATTAGGGATAAAAGAAAACGACCGCGGAA
GGACCTCATAATTGGATCACCATGTCAACTGATTACCATGTCTGAACGAGAGGAAGAGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11