Sequences from confirmation amplicon


Confirmation sequences for line 014H04 and hit At1g10230 are shown.
Line ID 014H04
Locus At1g10230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3355473
Primers used for PCR 03jx - 8474, show primer details
   
Sequence Name 65-K113379-0022-014-H04C-03jx
GenBank Accession LN511439 [GenBank]
Sequencing primer gene specific primer, 03jx: 5'-TTCTTCACTAGGTTCATTGACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113379-0022-014-H04C-03jx
TCCCCGGTGACATTTTCAGCGGAATTGCTTCACCGGCGCAGTTATCCTCCATCATGTGCA
CTATGATCAGAAACTTACGCGCCACCGCTTCGTCGATCTCGAAAGACTCGCCATCGGAAC
TCGTCAACAAAATCTTGTTAGAAGACATGGTTAGACTCGCAACCGCTTCCTCGATCTCTG
ATGATTCGCCGGCGGACACAATCTCTTCGGAAGAAGAAGCCATTGTTATAAGGCAAATGA
GTAATCCAGAATAAACCCTTACAAGTAGGGGAGAAAAGGAAATATAATGGATCAGAATTA
TGGGCCTAAAGATTTCATAAAGCCCAAACAACCTATACGGCTGTACTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATA
A
   
Sequence Name 27-K113377-0022-014-H04C-8409
GenBank Accession LN498472 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113377-0022-014-H04C-8409
TTCATTGAGTACAGCCGTATAGGTTGTTTGGGCTTTATGAAATCTTTAGGCCCATAATTC
TGATCCATTATATTTCCTTTTCTCCCCTACTTGTAAGGGTTTATTCTGGATTACTCATTT
GCCTTATAACAATGGCTTCTTCTTCCGAAGAGATTGTGTCCGCCGGCGAATCATCAGAGA
TCGAGGAAGCGGTTGCGAGTCTAACCATGTCTTCTAACAAGATTTTGTTGACGAGTTCCG
ATGGCGAGTCTTTCGAGATCGACGAAGCGGTGGCGCGTAAGTTTCTGATCATAGTGCACA
TGATGGAGGATAACTGCGCCGGTGAAGCAATTCCGCTTGAAAATGTCACCGGGGATATCC
TCTCCAAGATAATCGAGTACGCGAAGATGCACGTCAATGAACCTAGTGAAGA

Last Updated on 06.12.2018 16:11