Sequences from confirmation amplicon


Confirmation sequences for line 366G11 and hit At1g11572 are shown.
Line ID 366G11
Locus At1g11572 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3884376
Primers used for PCR 04ij - 8474, show primer details
   
Sequence Name 54-K113845-0022-366-G11C-04ij
GenBank Accession LN508544 [GenBank]
Sequencing primer gene specific primer, 04ij: 5'-AAAATCTTGATTTCTTTTTCCGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113845-0022-366-G11C-04ij
AGAACAACCAGATTGGCACGCAGGTTCCGCAAATGTCTTGCGTGGCTTTCGATTCACTTT
CATGCATATTGGCATACATTCTCTGTGGCATAGCGGCAATACTTTCTTTCCTAGTAGTGC
TTCTTGTCCTACTACGATATCCGACCATGTCAATAACGTAATTATCATCAAACTCAGGGT
GGCTAAGTTTTTGCTCCGGTTCTTCATATTGTAGTTCGTGAGAAGTTGATCAGGAAAAGG
GAGAGAGAGAAAAATGGTTTCGTGGAAGAGAAGGAAATGTTTTTGACTATAAATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 27-K113844-0022-366-G11C-8409
GenBank Accession LN498477 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113844-0022-366-G11C-8409
ATTTATAGTCAAAACCTTTTCCTTTCTCTTCCACGAAACCATTTTTTCTCTCTCTCCCTT
TTCCTGATCAACTTCTCACGAACTACAATATGAAGAACCGGAGCAAAAACTTAGCCACCC
TGAGTTTGATGATAATTACGTTATTGACATGGTCGGATATCGTAGTAGGACAAGAAGCAC
TACTAGGAAAGAAAGTATTGCCGCTATGCCACAGAGAATGTATGCCAATATGCATGAAAG
TGATCGAAGCCACGCAAGACATTTGCGAACCTGCATGCCAATCTGGTTGTCTTCAGCTTC
AAGGTAGAGGCACCGGACTTTCAGCTTCCGATCAAGGAGTCGATATGGTCATTGCATAAA
TTAATATCTTTACCGGAAAAAG

Last Updated on 06.12.2018 16:11