Sequences from confirmation amplicon


Confirmation sequences for line 148D10 and hit At1g12040 are shown.
Line ID 148D10
Locus At1g12040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4071933
Primers used for PCR 31mk - 8474, show primer details
   
Sequence Name 40-K114277-0022-148-D10C-31mk
GenBank Accession LN503787 [GenBank]
Sequencing primer gene specific primer, 31mk: 5'-GGAGACTGGCTTGGTGAATAGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114277-0022-148-D10C-31mk
CTGGTGATGGTGGTGGAGGACTTGGTGTTACTGGGGGATAGTAAACTGGTGATGGTGGTG
GAGGTAATTATGATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGACTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 10-K114276-0022-148-D10C-8409
GenBank Accession LN490756 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114276-0022-148-D10C-8409
CCATTTACAATTGAAATATCCATCATACTCACGCCATTTACATGAAGCCATTTAAATGTA
GCCAGTAACATACAGTCCACCACCACCATCACCAGTTTACTATCCCCAAGTAACACCAAG
TCCTCCACCACCATCGCCGCTATACTATCCCCCAGTAACACCAAGACCACCACCACCATC
ACCAGTTTACTATCCCCCAGTAACACCAAGTCCTCCACCACCATCAGCAGTTTACTATCC
CCCAGTAACACCAAGACCTCCACCACCATCACCAGTTTACTATCCATCGGAAACACAAAG
TCCTCCACCACCTACTGAGTACTACTATTCACCAAGCCAATCTCC

Last Updated on 06.12.2018 16:11