Sequences from confirmation amplicon


Confirmation sequences for line 202E04 and hit At1g12110 are shown.
Line ID 202E04
Locus At1g12110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4108140
Primers used for PCR 08fm - 8474, show primer details
   
Sequence Name 26-K111117-0022-202-E04C-08fm
GenBank Accession LN497984 [GenBank]
Sequencing primer gene specific primer, 08fm: 5'-GAATCCCTTTCTCGGAAATAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K111117-0022-202-E04C-08fm
GAGGCAGGTGTTTTGTCTGTAGTGAAACGATCAAATATCGTGTCGTAGCACCTCCACTAC
TTTACACCTTCACCGGGACAGACCCAAAGTTAGGCATAATTAAGAACCCTAGCGTCACAT
GCACGACACGTGCTTTGCGTGCTCGGTGCTTCATTAAATTTCTCTTTCGTTTGGGTCTCT
CTAAAAGGTAGTTGAAAACTAAAGAAAAAACCTTTTACACTTTTCCATAAATTCAAACTC
TTAACTTTTTTTTGCCTACACTATTTTAAAAGCAATTACGTATTTGTCATGCATGTGTGT
GCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 10-K111116-0022-202-E04C-8409
GenBank Accession LN490715 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K111116-0022-202-E04C-8409
ATCCTGCCGCCGCTGCCGCACACACATGCATGACAAATACGTAATTGCTTTTAAAATAGT
GTAGGCAAAAAAAAGTTAAGAGTTTGAATTTATGGAAAAGTGTAAAAGGTTTTTTCTTTA
GTTTTCAACTACCTTTTAGAGAGACCCAAACGAAAGAGAAATTTAATGAAGCACCGAGCA
CGCAAAGCACGTGTCGTGCATGTGACGCTAGGGTTCTTAATTATGCCTAACTTTGGGTCT
GTCCCGGTGAAGGTGTAAAGTAGTGGAGGTGCTACGACACGATATTTGATCGTTTTCACT
ACAGACAAAACACCTGCCTCCCTACCCTAACTTCTCGTCTCTTCTTCTTTATTAGTTTTA
TTTCCGAGAAAGGGATT

Last Updated on 06.12.2018 16:11