Sequences from confirmation amplicon


Confirmation sequences for line 163B02 and hit At1g12411 are shown.
Line ID 163B02
Locus At1g12411 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4225253
Primers used for PCR 03nm - 8474, show primer details
   
Sequence Name 30-K114574-0022-163-B02C-03nm
GenBank Accession LN499826 [GenBank]
Sequencing primer gene specific primer, 03nm: 5'-AGGAAGCAATCGAGTATGGACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114574-0022-163-B02C-03nm
GCGCATCAAGAGACGCTCCTCGCCAGACGAAAGCGCAGGGCTAGGCTAGTCTTTTTGTTT
GTTTGTTATGTCAAAAGTTTTAATCTTTATGTTATTGTGATTGTTACCTAATAAAATCAC
TGATTATTGTTTTTTTATTCCACCGATTATCTGATTTCTACAAACATTTTAAACTGCTGT
GTAAGATGTAAAGGAAGTGACTTCCCTGGAAAATTCAACATATAGTCGCCATTACAGAAG
CTGAAGCAGAACAAGACATGAACTCTTTGCTTATTTTTCTGTCTAGTGGGGGAAGTAACG
CGTATGTTGACTTGGGAGTAATGGTAGTACAACGTAAAATGGCTCAATAGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAAATATA
   
Sequence Name 27-K114573-0022-163-B02C-8409
GenBank Accession LN498501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114573-0022-163-B02C-8409
CATTTACATTGAGCCATTTTACGTTGTACTACCATTACTCCCAAGTCAACATACGCGTTA
CTTCCCCCACTAGACAGAAAAATAAGCAAAGAGTTCATGTCTTGTTCTGCTTCAGCTTCT
GTAATGGCGACTATATGTTGAATTTTCCAGGGAAGTCACTTCCTTTACATCTTACACAGC
AGTTTAAAATGTTTGTAGAAATCAGATAATCGGTGGAATAAAAAAACAATAATCAGTGAT
TTTATTAGGTAACAATCACAATAACATAAAGATTAAAACTTTTGACATAACAAACAAACA
AAAAGACTAGCCTAGCCCTGCGCTTTCGTCTTGGCGAGGAGCGTCTTCTTTGATGCGCGG
TGGTCTAACAATCTTATCAATAAGTCCATACTCG

Last Updated on 06.12.2018 16:11