Sequences from confirmation amplicon


Confirmation sequences for line 383E04 and hit At1g12520 are shown.
Line ID 383E04
Locus At1g12520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4267887
Primers used for PCR BW84 - 8474, show primer details
   
Sequence Name 08-K106136-0022-383-E04C-8409
GenBank Accession LN489738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106136-0022-383-E04C-8409
GTAGATTTCCTCATTTGTTTATGATATTTACTTTGTGATGTGTTTTGGCAGATTTCTTAG
TCTCAGCTGCAGTAGCAGAATTCAAAGGCCCTGACATTTTCGGGGTAGTCCGATTTGCTC
AGGTCAGTATGGAACTTGCAAGAATCGAAGCCAACTTTACCGGTTTGTCACCCGGAACCC
ACAGCTGGTGTATCAACGAGTATGGAGATCTCACAAACGGAGCAGCCAGCACAGGGAGCT
TGTACAATCCATTTCAAGATCAAACTGGCACAGAGGTTGGTGAAACGCATTCTTAGAAAA
CGAAAATGTCATCACAATCCTCTAAAAACTCTCTTTTTCTTCTGTCAGCCATTGGGAGAC
CTGGGAACACTAGAGGCAGACAAAAACGGAGAGGCCTTTTATTCGGGAAAGAAAGAGAAA
CTCAAGG
   
Sequence Name 08-K106135-0022-383-E04C-BW84
GenBank Accession LN489737 [GenBank]
Sequencing primer gene specific primer, BW84: 5'-ACCTTGAGTTTCTCTTTCTTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106135-0022-383-E04C-BW84
CCTCTGTGTTCCCAGGTCTCCAATGGCTGACAGAAGAAAAAGAGAGTTTTTAGAGGATTG
TGATGACATTTTCGTTTTCTAAGAATGCGTTTCACCAACCTCTGTGCCAGTTTGATCTTG
AAATGGATTGTACAAGCTCCCTGTGCTGGCTGCTCCGTTTGTGAGATCTCCATACTCGTT
GATACACCAGCTGTGGGTTCCGGGTGACAAACCGGTAAAGTTGGCTTCGATTCTTGCAAG
TTCCATACTGACCTGAGCAAATCGGACTACCCCGAAAATGTCAGGGCCTTTGAATTCTGC
TACTGCAGCTGAGACTAAGAAATCTGCCAAAACACATCACAAAGTAAATATCATAAAACA
AATGAGGAAATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATATCCGCAGCGTTATTAT

Last Updated on 06.12.2018 16:11