Sequences from confirmation amplicon


Confirmation sequences for line 230D10 and hit At1g13260 are shown.
Line ID 230D10
Locus At1g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4542231
Primers used for PCR JE05 - 8474, show primer details
   
Sequence Name 40-K025950-022-230-D10C-8409
GenBank Accession LN503578 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K025950-022-230-D10C-8409
ACAGCAAGTGTTTCTTATTTCCCTCCCCCCAAGCTCTTTCTCCTTCTTTCTATATATATT
ATACACACACACGTATACATATACACAACATAATTCACAACACAACACAAACACATTTCT
GTTTTCTCCATTGTTTCAAACCATAAAAAAAAACACAGATTAAATGGAATCGAGTAGCGT
TGATGAGAGTACTACAAGTACAGGTTCCATCTGTGAAACCCCGGCGATAACTCCGGCGAA
AAAGTCGTCGGTAGGTAACTTATACAGGATGGGAAGCGGATCAAGCGTTGTGT
   
Sequence Name 41-K025950-022-230-D10C-JE05
GenBank Accession LN503938 [GenBank]
Sequencing primer gene specific primer, JE05: 5'-CCTAGTCATGTTTCCGTTACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K025950-022-230-D10C-je5
ACGACGCCGTTTACTCTGCTCTAACTCTTCGTTATAAGTATGTTTCCTCAACATATCAAC
GATCTCAGATTTCGAATGAGAATTCAAGAAATCGACCTCGTCTTCGTCCATCTTCACGTC
TTTGAAATTTGTGACGGCGTCACGGCGACGGAACCTGTGAACCGCGACGTCGTAGGCACG
AGCGGCTTCGTCTTCTTCGTTGAATGTCCCGAGCCACACGCGCTGGTGTTTCTCGTAAAT
CTGAGCTCCCCATCTTCCGTTTGGTTGTGGCACCACACCTTTGTATTTTGACGACGGAAG
CTTCCTAGATTCAGCTTCTACGCCGTTCTCTGAATCTAACACAACGCTTGATCCGCTTCC
CATCCTGTATAAGTTACCTACCGACGACTTTTTCGCCGGAGTTATCGCCGGGGTTTCACA
GATGGAACCTGTACTTGTAGTACTCTCATCAACGCTACTCGATTCCATTTAATCTGTGTT
TTTTTTTATGGTTTGAAACAATGGAGAAAACAGAAATGTGTTTGTGTTGTGTTGTGAATT
ATGTTGTGTATATGTATACGTGTGTGTGTATAATATATATAGAAAGAAGGAGAAAGAGCT
TGGGGGGAGGGAAATAAGAAACACTTGCTGTTTGAGCAATGAGTAT

Last Updated on 06.12.2018 16:11