Sequences from confirmation amplicon


Confirmation sequences for line 244A08 and hit At1g13400 are shown.
Line ID 244A08
Locus At1g13400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4598136
Primers used for PCR MY02 - 8474, show primer details
   
Sequence Name 29-K023494-022-244-A08C-8409
GenBank Accession LN499118 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K023494-022-244-A08C-8409
ATTTTCGTCTTTGTTGATTGTTTGGAAACCAAAAAAGTTTTGATCTAATCATTAGACGTA
CATTTTTGTATTAATTTAGATAAATCTAATTAAGCTATTAATCTTTATTATTGAGTAGGA
GGGTTTAGAGAGAAGCAAACAAAAGATGGGAAAGAGTATGAATGTAGATTTTGTTCACTA
AAGTTCTTCAAATCTCAAGCTCTCGGTGGCCACATGAACCGCCACCGTCAAGGCAAGATC
CCATTATTCTCTATATTATGTATATACACTAATACACATATCTTATTTTTTTTGGGGTTG
GTGAAATAGAAAGAGAGACGGAGTCACTAAACAAAGCTCGTGAACTTGTTCTTCGCAACG
ATAGCTTTCCTCCTCATCAAGGACCTCCATCGTTTAGGTACTTTTAGTTTCTATTTTCTT
TAACAATGTTTTACTCATTTTACATTTGATATCTTACTATAAATATTGATGTAAATATAT
AGTTATCATCAAGGAGATGTGCATATAGGAGACCTAACACAATTTAAACCAATGATGTAC
CCACCGAGACATTTCTCACTCCCCGGCTCTTCCTCCATCCTCCAGCTGCAACCACCATAT
CTTTA
   
Sequence Name 30-K023494-022-244-A08C-MY02
GenBank Accession LN499555 [GenBank]
Sequencing primer gene specific primer, MY02: 5'-AAGACAGCGGAGGATAAAGATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K023494-022-244-A08C-MY02
TTGCAGCTGGAGGATGGAGGAAGAGCCGGGGAGTGAGAAATGTCTCGGTGGGTACATCAT
TGGTTTAAATTGTGTTAGGTCTCCTATATGCACATCTCCTTGATGATAACTATATATTTA
CATCAATATTTATAGTAAGATATCAAATGTAAAATGAGTAAAACATTGTTAAAGAAAATA
GAAACTAAAAGTACCTAAACGATGGAGGTCCTTGATGAGGAGGAAAGCTATCGTTGCGAA
GAACAAGTTCACGAGCTTTGTTTAGTGACTCCGTCTCTCTTTCTATTTCACCAACCCCAA
AAAAAATAAGATATGTGTATTAGTGTATATACATAATATAGAGAATAATGGGATCTTGCC
TTGACGGTGGCGGTTCATGTGGCCACCGAGAGCTTGAGATTTGAAGAACTTTAGTGAACA
AAATCTACATTCATACTCTTTCCCATCTTTTGTTTGCTTCTCTCTAAACCCTCCTACTCA
ATAATAAAGATTAATAGCTTAATTAGATTTATCTAAATTAATACAAAAATGTACGTCTAA
TGATTAGATCAAAACTTTTTTGGTTTCCAAACAATCAACAAAGACGAAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA

Last Updated on 06.12.2018 16:11