Sequences from confirmation amplicon


Confirmation sequences for line 343G02 and hit At1g13560 are shown.
Line ID 343G02
Locus At1g13560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4638618
Primers used for PCR PE74 - 8474, show primer details
   
Sequence Name 09-K022491-022-343-G02C-8409
GenBank Accession LN489992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K022491-022-343-G02C-8409
TTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCGAAAAAAATGCTCCTTCTA
AAATCATTTTCGTAGCATTTCAAGAATAGTTTTCGTATTTGCCCAATGCATAAATCCAAA
AACAAGAACATTATACAAAGTTCTACATAACTTCACCTATCCTGATCAAACAGACAACAA
TGAATAAGAAGAAGAAATACATCACCGATCCAACTGGTCCATATCGACCATTCGCAGTGT
CAAAGGTTTCTTTTCAAGCTTCTTTACGCGTGATCCTGTGGATTAGAAACCAGAGATATG
TACATTAATTCATTATGCTTTCAAAACAAGAATTTCGATCTTCTTTATCTAAATTTTCAC
CTAAAGCAGTAGATTCCAAGGGCCTCAGTGATCTCATGGATGACTGATGTTGCAAAGTGC
AAGTATAATGACACTGTTTTGCATCAATACAACATTGGTTTAGAGCGGGCAAAATAACTG
AATAGTGAAAAAGAAAAATCAACAGCTAACTAAGGCTTTTTTTCTCTTTAGGAGCAAACC
TGTGAATATGCAGTAGCCAAGAAGAACCCATAATTCGTCGACTAGAGGAACCCTGAAATA
TGAAGTTTCTCAGGGTGTTAAAGAAAAAAAAGAACCGATGAAGTTTGTGCGGAAA
   
Sequence Name 10-K022491-022-343-G02C-PE74
GenBank Accession LN490466 [GenBank]
Sequencing primer gene specific primer, PE74: 5'-TTTCTTTCTTTAAATTTTCCGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K022491-022-343-G02C-PE74
TTCATCGGTTCTTTTTTTTCTTTAACACCCTGAGAAACTTCATATTTCAGGGTTCCTCTA
GTCGACGAATTATGGGTTCTTCTTGGCTACTGCATATTCACAGGTTTGCTCCTAAAGAGA
AAAAAAGCCTTAGTTAGCTGTTGATTTTTCTTTTTCACTATTCAGTTATTTTGCCCGCTC
TAAACCAATGTTGTATTGATGCAAAACAGTGTCATTATACTTGCACTTTGCAACATCAGT
CATCCATGAGATCACTGAGGCCCTTGGAATCTACTGCTTTAGGTGAAAATTTAGATAAAG
AAGATCGAAATTCTTGTTTTGAAAGCATAATGAATTAATGTACATATCTCTGGTTTCTAA
TCCACAGGATCACGCGTAAAGAAGCTTGAAAAGAAACCTTTGACACTGCGAATGGTCGAT
ATGGACCAGTTGGATCGGTGATGTATTTCTTCTTCTTATTCATTGTTGTCTGTTTGATCA
GGATAGGTGAAGTTATGTAGAACTTTGTATAATGTTCTTGTTTTTGGATTTATGCATTGG
GCAAATACGAAAACTATTCTTGAAATGCTACGAAAATGATTTTAGAAGGAGCATTTTTTT
CCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATG
Primers used for PCR FN07 - 3144, show primer details
   
Sequence Name 37-K031938-022-343-G02C-3144
GenBank Accession LN502506 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K031938-022-343-G02C-3144
TATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGT
ACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACCATCA
TACTCATTGCTGATCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTATCTTG
TACCTCGAAAATTGGCTGGCTGGATTTGGTTTGCAAGCATTTCAGGAATTGGTTGACTTT
TGACTTTTTGTAATACGACTATACCAAAGAGTTCTGTATATCAGTGTCTCGCATTTTAAA
CCCTAAAGAAATTACATATTGAGTTCCTACTTCCTAACATCATATTAAAACATTAGTTAA
GAAGACTTAAATAAAAAAATCAACGCATAGTTTTGACCAACCAAAAGTCAACATATTTTT
GGAACCTACAAAATGGGCAATAACAATGTTCACACAATTCATAGAACAAACTATCATGTA
TGTAACAAGGCGTTTATCAGAGTATCGGAAAAACCCGTCGGAACTTAAGAAGCCTCCACC
GGAAGGTCCAAACTCCGGCGTACTTGTGATTCAAGACGAAGAATCGTTGACCACTTGTTG
CTTTGGTTTGTGCAACGAGATTTTCCTAAGAGGATTACCGNTTTCCACA
   
Sequence Name 38-K031938-022-343-G02C-FN07
GenBank Accession LN502878 [GenBank]
Sequencing primer gene specific primer, FN07: 5'-CTGTGGAAACGGTAATCCTCTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K031938-022-343-G02C-FN07
CACAGTGGTCAACGATTCTTCGTCTTGAATCACAAGTACGCCGGAGTTTGGACCTTCCGG
TGGAGGCTTCTTAAGTTCCGACGGGTTTTTCCGATACTCTGATAAACGCCTTGTTACATA
CATGATAGTTTGTTCTATGAATTGTGTGAACATTGTTATTGCCCATTTTGTAGGTTCCAA
AAATATGTTGACTTTTGGTTGGTCAAAACTATGCGTTGATTTTTTTATTTAAGTCTTCTT
AACTAATGTTTTAATATGATGTTAGGAAGTAGGAACTCAATATGTAATTTCTTTAGGGTT
TAAAATGCGAGACACTGATATACAGAACTCTTTGGTATAGTCGTATTACAAAAAGTCAAA
AGTCAACCAATTCCTGAAATGCTTGCAAACCAAATCCAGCCAGCCAATTTTCGAGGTACA
AGATAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGATCAGCAATGAGTATG
ATGGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTAC
CCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAG
TGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAA
GACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG

Last Updated on 06.12.2018 16:11