Sequences from confirmation amplicon


Confirmation sequences for line 050B04 and hit At1g14760 are shown.
Line ID 050B04
Locus At1g14760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5084461
Primers used for PCR BB73 - 8474, show primer details
   
Sequence Name 47-K104125-0022-050-B04C-BB73
GenBank Accession LN506188 [GenBank]
Sequencing primer gene specific primer, BB73: 5'-TCTTACACCATGTTAATGACCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K104125-0022-050-B04C-BB73
CTTAGTCACTTTTTATCTTTGTGTATAAATTTATGTACACAAAAATATGCAACAACCGTT
ACATAGTAAGAAAGGCAATGCGTTACAACTTACAAGATGACGCAATTATAAATGGTTATG
AAAAATAATCATGGAAGCTAGTGTTGTTTTTATATTTAAATTTTACTGTTGTTTTCTAAA
GAGTGTGACTAAATGATCAATGCAATATTTACAATATAATTAGTTTTATTTTTCTTAAAT
AAAAAAGCAGGAGGGCATGCCATAAATTACGGTTACCAATAAACGATCATACAAACGCGT
TACGTAAACGCAGTACAATAAATCGTGAGTAAACGCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 63-K104126-0022-050-B04C-8409
GenBank Accession LN510979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K104126-0022-050-B04C-8409
TGAGCCATTTACAATTGAATATATCCTCGCGTTTACTCACGATTTATTGTACTGCGTTTA
CGTAACGCGTTTGTATGATCGTTTATTGGTAACCGTAATTTATGGCATGCCCTCCTGCTT
TTTTATTTAAGAAAAATAAAACTAATTATATTGTAAATATTGCATTGATCATTTAGTCAC
ACTCTTTAGAAAACAACAGTAAAATTTAAATATAAAAACAACACTAGCTTCCATGATTAT
TTTTCATAACCATTTATAATTGCGTCATCTTGTAAGTTGTAACGCATTGCCTTTCTTACT
ATGTAACGGTTGTTGCATATTTTTGTGTACATAAATTTATACACAAAGATAAAAAGTGAC
TAAGCTTAAAATATCCTTGAAAAAGCCTTTGGGTCATTAAATGGNTGTAA

Last Updated on 06.12.2018 16:11