Sequences from confirmation amplicon


Confirmation sequences for line 780B02 and hit At1g15130 are shown.
Line ID 780B02
Locus At1g15130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5206639
Primers used for PCR 01ae - 8474, show primer details
   
Sequence Name 75-K106292-0022-780-B02C-8409
GenBank Accession LN513247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K106292-0022-780-B02C-8409
GAGCCATTTACATGTATAAACGTCAGTCTTTTTCTTTTTCCTTTGTCATCACAAAATCAC
TGCACTGCTGCTTCACATTCGTGATTGCATCCTATTGAGACCAACAAAGACAATGTCTCA
GATTGCAGGAAAGTTATTTCGTATCTTCAATGGAGTGTAAAATCAGAATAGAAGAACCAT
CCCGGACAAGTGGCATAATCTAGACAAGCTTTGACAGTGAAGAGCTTACTACTATATAGA
GAAAAGAAACTCACTTGGAGAGTGACATAGAACTTCAGGCCCTCATTGATGTTCTCCTTG
ATCTCTCGATACTTCATTATTGCAGCTTGAATCTGCTTGTAGCACTTCTCCTTGGATGCT
GAACAACAGAGAAACACAGAAAACGAATGTCTGAGAGCAAAAGAACCGAAATACGGGAGC
ATAAGAAATTCCACTACTTCAAAGCCGAATGTGTCAGTTCTATCATGGATTATACTTCAA
CCATCGTATAGAATGCAATACAGAAAGTACTTTGATCCATCATGCAAGACAGAAGCTGCT
TGCTCACCTTTATAATCTTCAAGATTGAAAATAGTTGAAAACTCTC
   
Sequence Name 75-K106293-0022-780-B02C-01ae
GenBank Accession LN513248 [GenBank]
Sequencing primer gene specific primer, 01ae: 5'-GGCTCAAAATGAAGAGTTTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K106293-0022-780-B02C-01ae
TAAGGTGAGCAGCAGCTTCTGTCTTGCATGATGGATCAAGTACTTTCTGTATTGCATTCT
ATACGATGGTTGAAGTATAATCCATGATAGAACTGACACATTCGGCTTTGAAGTAGTGGA
ATTTCTTATGCTCCCGTATTTCGGTTCTTTTGCTCTCAGACATTCGTTTTCTGTGTTTCT
CTGTTGTTCAGCATCCAAGGAGAAGTGCTACAAGCAGATTCAAGCTGCAATAATGAAGTA
TCGAGAGATCAAGGAGAACATCAATGAGGGCCTGAAGTTCTATGTCACTCTCCAAGTGAG
TTTCTTTTCTCTATATAGTAGTAAGCTCTTCACTGTCAAAGCTTGTCTAGATTATGCCAC
TTGTCCGGGATGGTTCTTCTATTCTGATTTTACACTCCATTGAAGATACGAAATAACTTT
CCTGCAATCTGAGACATTGTCTTTGTTGGTCTCAATAGGATGCAATCACGAATGTGAAGC
AGCAGTGCAGTGATTTTGTGATGACAAAGGAAAAAGAAAAAGACTGACGTTTATACATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR 35cs - 8474, show primer details
   
Sequence Name 52-K108477-0022-780-B02C-35cs
GenBank Accession LN507841 [GenBank]
Sequencing primer gene specific primer, 35cs: 5'-GGCTCAAAATGAAGAGTTTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K108477-0022-780-B02C-35cs
AGGTGAGCAGCAGCTTCTGTCTTGCATGATGGATCAAGTACTTTCTGTATTGCATTCTAT
ACGATGGTTGAAGTATAATCCATGATAGAACTGACACATTCGGCTTTGAAGTAGTGGAAT
TTCTTATGCTCCCGTATTTCGGTTCTTTTGCTCTCAGACATTCGTTTTCTGTGTTTCTCT
GTTGTTCAGCATCCAAGGAGAAGTGCTACAAGCAGATTCAAGCTGCAATAATGAAGTATC
GAGAGATCAAGGAGAACATCAATGAGGGCCTGAAGTTCTATGTCACTCTCCAAGTGAGTT
TCTTTTCTCTATATAGTAGTAAGCTCTTCACTGTCAAAGCTTGTCTAGATTATGCCACTT
GTCCGGGATGGTTCTTCTATTCTGATTTTACACTCCATTGAAGATACGAAATAACTTTCC
TGCAATCTGAGACATTGTCTTTGTTGGTCTCAATAGGATGCAATCACGAATGTGAAGCAG
CAGTGCAGTGATTTTGTGATGACAAAGGAAAAAGAAAAAGACTGACGTTTATACATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTA
   
Sequence Name 36-K108476-0022-780-B02C-8409
GenBank Accession LN502253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K108476-0022-780-B02C-8409
TCGGAATGAGCCATTTACAATGTATAAACGTCAGTCTTTTTCTTTTTCCTTTGTCATCAC
AAAATCACTGCACTGCTGCTTCACATTCGTGATTGCATCCTATTGAGACCAACAAAGACA
ATGTCTCAGATTGCAGGAAAGTTATTTCGTATCTTCAATGGAGTGTAAAATCAGAATAGA
AGAACCATCCCGGACAAGTGGCATAATCTAGACAAGCTTTGACAGTGAAGAGCTTACTAC
TATATAGAGAAAAGAAACTCACTTGGAGAGTGACATAGAACTTCAGGCCCTCATTGATGT
TCTCCTTGATCTCTCGATACTTCATTATTGCAGCTTGAATCTGCTTGTAGCACTTCTCCT
TGGATGCTGAACAACAGAGAAACACAGAAAACGAATGTCTGAGAGCAAAAGAACCGAAAT
ACGGGAGCATAAGAAATTCCACTACTTCAAAGCCGAATGTGTCAGTTCTATCATGGATTA
TACTTCAACCATCGTATAGAATGCAATACAGAAAGTACTTTGATCCATCATGCAAGACAG
AAGCTGCTTGCTCACCTTTATAATCTTCAAGATTGAAAATAGTTGAAAACTCTTCATTTT
GAGC

Last Updated on 06.12.2018 16:11