Sequences from confirmation amplicon


Confirmation sequences for line 065A10 and hit At1g15240 are shown.
Line ID 065A10
Locus At1g15240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5248512
Primers used for PCR 47ym - 8474, show primer details
   
Sequence Name 82-K120751-0022-065-A10C-47ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47ym: 5'-AGTGTTCAAAATTGGTCCCCTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K120751-0022-065-A10C-47ym
ATACTGTATGCCAGTTGACTCGAGGTAATATCAGAACCAGGCCCATGAAGCCCATTAAGA
AGCCCATATTTTTGGCGGTAACCAATGAAGATCCATTTTGGGTAGGAAAAACCCTAAAGG
ACGCTACACAGTCGTCTTCTCGGAGCCGCAAAGATTTCTGTAGAGCAAACTTTGATCATC
CCCTGCATCTGCGGCAATGGTAATCTATTGGCATTTTTTTTCCATGTAAGCTCATGTGTG
ACTTATGTGTAACCGGTGCTTTGGAAATGACAGGGTAAAGGAACAGGGAGCTTTGGTAAG
AGGAGGAACAAGTCACACACGCTCTGTGTGAGATGTGGTCGCCGGAGCTTTCACATCCAG
AAGAGTCGTTGCTCGGCATGTGCTTATCCAGCTGCCAGAAAGAGGACATGTATGTACTCT
CTCTCTATTTCTGTCTCTGTCTTGGAATTTTGGGTCTGATCATGGAGAATTGGAGATATA
TGAAAGAGTCCTGGAATTTATGATTTTGTGTGCAGATAACTGGAGTGTGAAGGCAATAAG
AAGAAAGACAACAGGAACTGGTAGGATGAGGTATCTCCGTAATGTCCCTCGAAGGTTCAA
GACTTGCTTTAGAGAAGGTAAAGCTACATTGCACCCTTTGCTTCACTTAAATATTTGATA
TAATCTAAGACTTGCTCAATCTGATCGATGGAATTCTGTGAATCAGGTACGCAAGCCACC
CCAAGAAACAAAGCTGCAGCTTCGTCTTCCTAGGCATTGTTCAGTTTGCGTTTTGCCATC
TCTTTCTTGCTATGTAGCTTTTTTTT
   
Sequence Name 68-K120750-0022-065-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K120750-0022-065-A10C-8409
GCCATTTACAATTGAAGCCATTGCTACTATCAGCGTTTCCGGAAATGGAGCAAGCTTTCA
AAAAATTGCACTACGAAAAACACCTCTTTGGTCAATATACACCACCGAACTGATACTTGG
ATTTTCATTGTAATCATCTTCCATATTACGTGGAGTTTTGCTAGAATTCTTTAAACAGTA
ATAGCACGGATGCATTTCATTGATGAGTGATCACACTATCAGAAATAGGATTGCACAGTA
AAGGAAGTACAAGAGCAAAAAAGATAAACAAACGAATTAAAAAAAAAAGCTACATAGCAA
GAAAGAGATGGCAAAACGCAAACTGAACAATGCCTAGGAAGACGAAGCTGCAGCTTTGTT
TCTTGGGGTGGCTTGCGTACCTGATTCACAGAATTCCATCGATCAGATTGAGCAAGTCTT
AGATTATATCAAATATTTAAGTGAAGCAAAGGGTGCAATGTAGCTTTACCTTCTCTAAAG
CAAGTCTTGAACCTTCGAGGGACATTACGGAGATACCTCATCCTACCAGTTCCTGTTGTC
TTTCTTCTTATTGCCTTCACACTCCAGTTATCTGCACACAAAATCATAAATTCCAGGACT
CTTTCATATATCTCCAATTCTCCATGATCAGACCCAAAATTCCAAGACAGAGACAGAAAT
AGAGAGAGAGTACATACATGTCCTCTTTCTGGCAGCTGGATAAGCACATGCCGAGCAACG
ACTCTTCTGGATGTGAAAGCTCCGGCGACCACATCTCACACAGAGCGTGTGTGACTTGTT
CCTCCTCTTACCAAAGCTCCCTGTTCCTTTACCCTGTCATTTCCAAAGCACCG

Last Updated on 06.12.2018 16:11